Concanavalin characteristics

Muller, A.J. and Carr, P.W., Chromatographic study of the thermodynamic and kinetic characteristics of silica-bound concanavalin A, J. Chromatogr., 284, 33-51, 1984. 

The characteristics of Ca2+-independent release are peculiar it requires the presence of divalent cations, such as Mg2+, which can be added or removed in succession, causing respective bouts of secretion or its cessation (Misler and Hurlbut 1979). In the absence of Mg2+, this release can be supported by slightly hypertonic sucrose, by itself insufficient to cause secretion (Misler and Hurlbut 1979). The Ca2+-independent release can be blocked by millimolar La3+ (Rosenthal et al. 1990) or concanavalin A (Grasso et al. 1978 Boehm and Huck 1998). It may involve release of Ca2+ from mitochondria, as observed in peripheral (Tsang et al. 2000) and not central synapses (Adam-Vizi et al. 1993), but it is unclear if stored Ca2+i itself can trigger release. 

Stern M, Gellermann B. Food proteins and maturation of small intestinal microvillus membranes (MVM). I. Binding characteristics of cows milk proteins and concanavalin A to MVM from newborn and adult rats. J Pediatr Gastroenterol Nutr 1988 7 115-121. 

Arabinogalactan proteins (AGP) represented a major proportion (40%) of the total soluble polysaccharides (300 mg/1) in a red Carignan wine (Pellerin etal., 1995 Vidal etal., 2001). These macromolecules were first fractionated on a DEAF Sephacel anion exchanger, then separated from the yeast mannoproteins by affinity chromatography on Concanavaline A and finally purified, by exclusion chromatography on a Sephacryl S400 column. Table 3.4 shows the molecular characteristics of the five species obtained. 

Investigation of the binding characteristics of urinary acid 3-D-gaIactosidase, 3-D-2-acetamido-2-deoxyglucosidase, a-D-galactosidase and a-L-fucosidase from patients with mucolipidosis II and mucolipidosis III to agarose-immobilized concanavalin A revealed a two to ten-fold decrease in the proportion of enzyme (that adsorbed on the lectin) activities from patients with mucolipidoses II and III. Neuraminidase treatment of the unabsorbed enzyme fraction did not... 

D-Glucose and myristic, palmitic, and stearic acids, as well as d-(—)-glyceric acid, are components of a glycolipid isolated from Nocardia caviae. Wheat-germ agglutinin and concanavalin A have been used in studies of the binding characteristics of mycoplasma cells and membranes. ... 

An affinant for neuraminidase, which was formed by reaction of ai-acid glycoprotein with agarose cyclic imidocarbonate, did not display non-specific adsorption that is characteristic of the analogous oxamic acid derivatives. Concanavalin A immobilized on agarose cyclic imidocarbonate effected different bindings, depending on whether or not ethylene glycol was present. ... 

Investigations of the binding of human-fibroblast 104 interferon to concanavalin A and the affinity-chromatography characteristics of immobilized concanavalin A... 

Because of our interest in utilizing Cd NMR spectroscopy as a probe for calcium sites in biological systems (42,43,49,51), we have been interested in the isotropic Cd chemical shifts in these compounds. In the systems studied (Concanavalin A (42), Parvalbumin (43), Troponin C (50),. Calmodulin (49), and Insulin (51)), replacing calcium with cadmium places cadmium in an environment in which all of the atoms in the first coordination sphere are oxygens. The cadmium is assumed to be six coordinate. The isotropic chemical shifts for these compounds (82) fall into a characteristic range of chemical shifts from -85 to -130 ppm with respect to 0.1 M Cd(C104)2 To date, there are no model compounds that can be studied in aqueous solutions which have isotropic shifts within this range. 

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