Comprehensive screens

There is no single library creation method that will satisfy the ideal requirements of high-diversity quality, large library size, and ultimately the feasibility of comprehensive screening of the generated library. Evaluation of each case is necessary to determine which library creation method is suitable for the project s goals. 

As more and more enzymes become available, it is essential to develop an automated microtiter-based screening protocol, which allows the rapid identification of desired biocatalyst hits from a family of enzyme libraries using a minimal amount of substrates and enzymes. As a result of comprehensive screening, the success rate can be significantly improved and many unique enzymes or conditions can be identified, which were previously largely ignored in the synthetic community (Yazbeck et al. 2003). 

In a comprehensive screen of the effect of TADDOL and BINOL-derived phosphites on the conjugate addition of diethylzinc to representative enones, Al-exakis et al. (181) revealed that the Feringa ligand exhibits the broadest scope, providing the conjugate adducts in selectivities ranging from 66 to 92% ee, Eq. 144. 

Primary metabolites, co-factors, and marketed drugs form additional sets of biologically relevant and validated compounds that constitute an essential component of a comprehensive screening collection. 

Ho, E.N.M., Leung, D.K.K., Wan, T.S.M. and Yu, N.H. (2006) Comprehensive Screening of Anabolic Steroids, Corticosteroids, and Acidic Drugs in Horse Urine by Solid-Phase Extraction and Liquid Chromatography-Mass Spectrometry. J Chromatopfr A, 1120, 38. 

Pitt JJ, Eggington M, Kahler SG (2002) Comprehensive screening of urine samples for inborn errors of metabolism by electrospray tandem mass spectrometry. Clin Chem 48 1970-1980... 

Hudson JC, Golin M, Malcolm M. Capillary zone electrophoresis in a comprehensive screen for basic drugs in whole blood. J Can Soc Forensic Sci 1995 28 137. 

We need to calculate the economic costs and benefits of comprehensive screening of newborns. We must also consider the potential consequences—such as emotional ones—of genetic disease on the family and society the potential loss of privacy to tested individuals and the use and abuse of the information by schools, employers, insurance companies, and other private or governmental agencies. 

During our work on enzymatic nitrile transformation, carbocyclic y-amino nitriles have emerged from a comprehensive screening among structurally diverse amino nitriles as being weU-suited substrates for nitrilase-mediated hydrolysis. In contrast, as stated in the previous section, the analogous carbocychc 5-amino nitriles are strictly non-substrates for nitrilases. 

This technique, when combined with gas chromatography, provides the most comprehensive screening method available, with high specificity and sensitivity, Mid wide applicability. It can be used for identification and quantification down to very low concentrations, using the technique of selected ion monitoring whereby tiie mass specti ometer acts as a sensitive and specific detector for the gas chromatograph. Further information on this technique may be found under Mass Specti-ometiy (p. 259). 

A comprehensive screening program evaluated the capability of various aqueous systems to scrub NOa- from the flue gases emitted by stationary power plants flred with fossil fuels. These are the conclusions of this study ... 

Abrams, E.S., S.E. Murdaugh and L.S. Lerman. Comprehensive screening of the human KRAS2 gene for sequence variants. Genes Chromosomes Cancer 6 73-85, 1993. 

Reproduced from the Journal of Analytical Toxicology by permission of Preston Publications, A Division of Preston Industries, Inc. Sharp M.E., A comprehensive screen for volatile organic compounds in biological fluids, J. Anal. Toxicol. 2001, 25, 631-636. 

Cooper, S.F. Masse, R. Dugal, R. Comprehensive screening procedme for diuretics in urine by high-performance liquid chromatography. J.Chromatogr., 1989, 489, 65—88... 

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