Complementarity determining regions region

Cacia, J., Keck, R., Presta L. G., and Frenz, J., Isomerization of an aspartic acid residue in the complementarity-determining regions of a recombinant antibody to human IgE identification and effect on binding affinity, Biochemistry, 35, 1897, 1996. 

Bevacizumab is produced using CHO expression in a nutrient medium with gentamicin antibiotic. It has a molecular weight of 149 kDa. The antibody is humanized, with human framework and murine complementarity-determining regions. 

The differences in sequence within the variable region produce a practically unlimited number of possible three-dimensional arrangements for the amino acid side chains to form the complementarity-determining region (CDR), which actually binds to the antigen. 

Guillaume, T., Rubinstein, D.B., Young, F., et al. (1990). Individual VH genes detected with oligonucleotide probes from the complementarity-determining regions. J. Immunol., 145, 1934-1945. 

Jones, P.T., Dear, P.H., Foote, J., et al. (1986). Replacing the complementarity-determining regions in a human antibody with those from a mouse. Nature, 321, 522-525. 

Raaphorst, F.M., Timmers, E., Renter, M.J.H., et al. (1992). Restricted utilization of germline VH3 genes and short diverse third complementarity-determining regions (CDR3) in human fetal B-lymphocyte immunoglobulin heavy chain rearrangements. Eur. J. Immunol., 22, 247-251. 

---