Collision-induced dissociation efficiency

A mass range of 450 amu with unit mass resolution was obtained. Limits of detection for trace analysis in air of methyl salicylate (1.24 ppb) and for nitrobenzene (629 ppt) were achieved. Isolation and collision-induced dissociation efficiencies in MS/MS experiments were greater than 50%. 

Fragmentor voltage Over 220 V Under 90 V 110-150V Collision-induced dissociation effect Poor ionization efficiency Good ionization efficiency... 

G. P. Jackson, F. I. King, D. C. Duckworth, Efficient polyatomic interference reduction in plasma source mass spectrometry via collision induced dissociation, J. Anal. Atom. Spectrom., 18 (2003), 1026-1032. 

The on-line coupling of CE with electrospray ionization mass spectrometry (CE-ESI-MS) allows high separation efficiency together with high sensitivity and selectivity as well as molecular structural information. A CE-UV-ESI-MS method was developed for the analysis of hoscyamine, scopolamine, and other tropane derivatives [131]. The differentiation of hyoscyamine from littorine, commonly encountered in plant material, was demonstrated using in-source collision-induced dissociation. The developed method was applied to the analysis of these alkaloids in Belladonna leaf extract and in Datura Candida x D. awreahairy root extract. Recently, CE coupled with electrochemiluminescence detection has been used for the determination of atropine and scopolamine in Flos daturae [132]. 

The collision-induced dissociation (CID) spectrum of the m/z 94 ions, recorded at 20 eV kinetic energy, features only three peaks associated with the losses of H (m/z 93, 12%), NH3 (m/z 77, 86%) and [NH3+C2H2] (m/z 51, 1.5%) see Figure 13a. The very efficient ammonia loss confirms that we are dealing with the IV-protonated... 

Efficient conversion of translational kinetic energy into internal energy has also been discovered for endothermic processes other than atom transfer, such as charge transfer [176—179], dissociative charge transfer [94,176, 180,181] and collision-induced dissociation [98, 169]. 

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