Datura Candida

Robins, R. J., Parr, A. J., Payne, J., Walton, N. J. and Rhodes, M. J. C. 1990. Factors regulating tropane alkaloid production in a transformed root culture of a Datura Candida x Datura aurea hybrid. Planta, 181 414-422. 

The on-line coupling of CE with electrospray ionization mass spectrometry (CE-ESI-MS) allows high separation efficiency together with high sensitivity and selectivity as well as molecular structural information. A CE-UV-ESI-MS method was developed for the analysis of hoscyamine, scopolamine, and other tropane derivatives [131]. The differentiation of hyoscyamine from littorine, commonly encountered in plant material, was demonstrated using in-source collision-induced dissociation. The developed method was applied to the analysis of these alkaloids in Belladonna leaf extract and in Datura Candida x D. awreahairy root extract. Recently, CE coupled with electrochemiluminescence detection has been used for the determination of atropine and scopolamine in Flos daturae [132]. 

Capsicum annum (chili pepper) Datura Candida (angel s trumpet) Datura stramonium (Jimson weed) Datura suaveolens (angel s trumpet) Lycium barbarum (Chinese wolfberry)... 

Angei s trumpet Datura Candida Feiwort Swertia species... 

Finlay P. Anticholinergic poisoning due to Datura Candida. Trop Doct 1998 28(3) 183-4. 

Hairy roots are not as readily manipulated by altering culture conditions or pH as are suspension cultures. However, the effect of temperature on growth and hyoscyamine production in transformed root cultures of Datura stramonium has been demonstrated by Hilton and Rhodes [86]. Another way to enhance the secondary metabolite accumulation of hairy roots is the addition of precursors and/or metabolic intermediates to the growth medium. The addition of (R,S)- phenyllactic acid increased significantly the accumulation of hyoscyamine and scopolamine in the hairy root culture of Datura Candida x D. aurea [72]. 

Alkaloids of a Datura Candida cultivar, D. aurea and various hybrids Fitoterapia 61(2) 148-152. 

Griffith, W.J. 1976. Agronomic evaluation of Datura Candida—A. new source of hy-oscine Economic Botany 30(4) 361—369. 

Hyoscyamine 6P-hydroxylase (H6H, EC 1.14.11.11) is a 2-oxoglutarate-dependent dioxygenase and is the final enzyme of the tropane alkaloid biosynthetic pathway. The H6H catalyzes the conversion of hyoscyamine into 6P-hydoxyhyoscyamine and scopolamine by two sequential reactions [24, 25]. Firstly, it catalyses the 6P-hydroxylation of hyoscyamine and secondly, the formation of the epoxy group leading to scopolamine [13]. Several works have revealed that the epoxidation activity is much lower than the hydroxylation one [11, 26]. These findings are in agreement to those reported in our laboratory about the H6H enzyme isolated from Brugmansia Candida (syn. Datura Candida) [27-29]. This South America n native plant is a natural hybrid between B. aurea and B. versicolor and a tropane alkaloids producer [30,31]. 

Christen P et al (1989) High-yield production of tropane alkaloids by hairy-root cultures of a Datura Candida hybrid. Plant Cell Rep 8 75-77... 

Christen P, Kapetanidis I (1987) Phytochemical study on the leaves of Lycium halimifolium Miller. Part 1. Studies on alkaloids. Pharmac Acta Helv 62 154-157 Christen P, Roberts MF, PhiUipson JD, Evans WC (1990) Alkaloids of hairy root cultures of a Datura Candida hybrid. Plant Cell Rep 9 101-104 Christen P, Roberts MF, Phillipson JD, Evans WC (1993) Alkaloids of Erythmxylum zambesiacum stem-bark. Phytochemistry 34 1147-1151... 

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