Collision-induced dissociation fragmentation efficiency

Figure 3.5 Nomenclature for peptide sequencing. Fragments resulting from backbone cleavages are mainly observed when MALDI peptide ions are fragmented in low-energy collision-induced dissociation (CID) tandem mass spectrometers. Of these, the cleavage at the amide bond will be most efficiently cleaved, resulting in y- and b-ions, depending on which part of the peptide will...

Such mass spectrometers are highly flexible since the possible combinations of sectors and the number of field-free regions allow many different experiments to be performed [142]. The limitation is that the fragmentation process becomes less probable with increasing mass and the upper limit seems to be around 2500 amu. To increase the dissociation efficiency not only collision cells, but also photo-dissociation and surface-collision-induced dissociation have been investigated as well as simultaneous recording with a zoom lens integrated into the second MS [143],... 

Fragment ions produced by up-front collision induced dissociation are very efficiently transported into the mass analyzer. The major advantage of this poor man s MS/MS method is its simplicity Only one voltage has to be increased, and no switching and adjustment of... 

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