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Cloning system Flexi

ORFs compatible with the Flexi cloning system can be easily ttansfetted between exptession vectots with diffetent antibiotics (ampicillin-tesistant and kanamycin-tesistant vectots) (tttNote 2)... [Pg.29]

Blommel PG, Martin PA, Wrobel RL, Steffen E, Fox BG. (2006) Hi efficiency single step production of expression plasmids from cDNA clones using the Flexi Vector cloning system Protein Expr Purif 47, 562-570. [Pg.40]

Here we present the protocols for cloning of ORFs from cDNA clones into the Flexi system by a PCR method and an ORF trap method and transferring them to expression vectors in a 96-well format. [Pg.27]

See other pages where Cloning system Flexi is mentioned: [Pg.6]    [Pg.7]    [Pg.8]    [Pg.9]    [Pg.9]    [Pg.10]    [Pg.25]    [Pg.26]   
See also in sourсe #XX -- [ Pg.5 , Pg.6 , Pg.7 , Pg.8 , Pg.26 , Pg.27 , Pg.29 ]



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