Cloning purification

Baitsch D, C Sandn, R Brandsch, GL Igloi (2001) Gene cluster on pAOl of Arthrobacter nicotinovorans involved in degradation of the plant alkaloid nicotine cloning, purification, and characterization of 2,6-dihydroxypyridine 3-hydrolase. J Bacterid 183 5262-5267. 

Tehara SK, JD Keasling (2003) Gene cloning, purification, and characterization of a phosphodiesterase from Delftia acidovorans. Appl Environ Microbiol 69 504-508. 

K. Goyal, P. Selvakumar, and K. Hayashi. 2001. Molecnlar cloning, purification and characterization of thermostable P-glucosidase from hyperthermophilic strain Thermotoga maritima activity in the presence of alcohols. J. Mol. Catal. B-Enzym. 15 45-53. 

Qiao F, Xing K, Eiu A, Ehlers N, Raghavachari N, Lou ME. Human lens thioltranferase cloning, purification, and function. Invest Ophthalmol Vis Sci 2001 42 743-751. 

Christgau, S., Kauppinen, S, Vind, J., Kofod, L.V., Dalboge, H., (1994). Expression cloning, purification and characterization of a 3-l,4-mannanase from Aspergillus aculeatus. Biochem Mol Biol Int 33 917-925. 

Parmar A, Kumar H, Marwaha S et al. (2000) Advances in enzymatic transformation of penidllins to 6-aminopenidllanic add (6-APA). Biotechnol Adv 18 289—301 Pasamontes L, Haiker M, Wyss M et ak (1997) Gene cloning, purification, and characterization of a heat-stable phytase from the fungus Aspergillus fumigatus. Appl Einviron Microbiol 63(5) 1696-1700... 

L., Clemente-Jim6nez, J.M., and Rodriguez-Vico, F. (2004) Molecular cloning, purification, and biochemical characterization of hydantoin racemase... 

Devine, J. H., et al. (1993). Luciferase from the east European firefly Luciola mingrelica cloning and nucleotide sequence of the cDNA overexpression in Escherichia coli and purification of enzyme. Biochim. Bio-phys. Acta 1173 121-132. 

Illarionov, B. A., et al. (2000). Recombinant obelin cloning and expression of cDNA, purification, and characterization as a calcium indicator. Method. Enzymol. 305 223-249. 

Bacterial bioluminescence, 30-46 factors required, 31 general scheme, 32 in vivo luminescence, 41 luminescence reaction, 37, 38 Bacterial luciferase, 33-35, 343 assay, 39 cloning, 34 crystal structure, 34 extraction and purification, 34 inactivation, 34, 35 molecular weight, 34 properties, 34 storage, 35 subunits, 34... 

Cypridina luciferase, 62-64, 343 cloning, 63 inhibitors, 63 molecular weight, 63 properties, 63, 64 purification, 62 turnover rate, 68... 

Until then, the purification of the Fepr protein had been a laborous job as a 240-L batch yielded only as little as 5 mg of protein. With the overexpression clones of the Fepr proteins, the range of proteinconsuming studies such as Mossbauer spectroscopy, EXAFS, and, last but not least, crystallization experiments was greatly extended. Thus, several groups set off to systematically investigate the spectroscopic properties of both Fepr proteins, poised at all four (proposed) redox states. 

The discovery and exploitation of enzymes in aldoxime-nitrile pathway nitrile hydratase, amidase, nitrilase, aldoxime dehydratase, etc., are shown along with the use of methodologies, such as organic chemistry, microbial screening by enrichment and acclimation culture techniques, enzyme purification, gene cloning, molecular screening by polymerase chain reaction (PCR). 

Here we describe the purification of RHG, the cloning and characterization of the corresponding gene and (over)production of the enzyme in A. aculeatus and Aspergillus awamori. 

Wu J-F, C-W Sun, C-Y Jiang, Z-P Liu, S-J Liu (2005) A novel 2-aminophenol 1,6-dioxygenase involved in the degradation of p-chloronitrobenzene by Comamonas strain CNB-1 purification, properties, genetic cloning and expression in Escherichia coli. Arch Microbiol 183 1-8. 

Furukawa K, N Arimura (1987) Purification and properties of 2,3-dihydroxybiphenyl dioxygenase from polychlorinated biphenyl-degrading Pseudomonas pseudoalcaligenes and Pseudomonas aeruginosa carrying the cloned bphC gene. J Bacterial 169 924-927. 

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