Chromosomal Integration Site

In each of these studies dozens of transgenic animals were made and characterized. This is necessary because there is considerable individual variability in phenotype, presumably because of different sized transgene concatemers and different chromosomal integration sites. In some foun-... 

In addition, mobile genetic elements have been described in both prokaryotes and eukaryotes. These segments of DNA can move from one chromosomal integration site to another, apparently unrelated to developmental processes. These processes represent a specialized form of recombination. 

Kotin, R. M., Menninger, J. C., Ward, D. C. and Bems, K. I. (1991). Mapping and direct visualization of a region-specific viral DNA integration site on chromosome 19ql3-qter. Genomics 10, 831-834. 

When a virus penetrates a cell instead of causing a productive infection it may transform that cell. For this to happen its genome (or a part of its genome) must integrate into the cellular chromosomes. The site of integration does not appear to be unique and often several copies of the viral DNA are integrated. 

Fig. n.4 FISH analysis of BC6 transgene integration sites carried by offspring of the 155-92 founder. Integrations located on chromosome 5 (1), chromosome 12 (2), chromosome 1 (3), and chromosome 23 (4) are respectively represented. 

The A, chromosome integrates at a specific site on the E. coli chromosome, attB, which maps between genes involved in galactose utilization and biotin synthesis (the gal and bio markers), as illustrated in Figure 25.17. Integration occurs at a specific site on the phage chromosome called attP. 

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