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Chromatography interaction ... hydroxyapatite

Ion-exchange, hydrophobic-interaction chromatography chromatography on hydroxyapatite and different types of affinity and pseudo-affinity chromatography are the most frequently used methods for isolation of nondenatured, physiologically active proteins. [Pg.179]

Interest in the nature of interactions between shortchain organic surfactants and large molecular weight macromolecules and ions with hydroxyapatite extends to several fields. In the area of carles prevention and control, surfactant adsorption plays an important role in the Initial states of plaque formation (1-5) and in the adhesion of tooth restorative materials ( ). Interaction of hydroxyapatite with polypeptides in human urine is important in human biology as hydroxyapatite has been found as a major or minor component in a majority of kidney stones ( 7). Hydroxyapatite is used in column chromatography as a material for separating proteins (8-9). The flotation separation of apatite from... [Pg.311]

Fractionation is less specific and essentially requires separation efficiency. This is generally performed with ion-exchangers and hydrophobic interaction chromatography, which enable efficient separation by salt or pH gradients. Hydroxyapatite is also a good tool for fractionation where proteins can be separated by both phosphate gradients and sodium chloride gradients. [Pg.605]

Hydroxyapatite Hydroxyapatite, which has the formula of Cajo(P04)6 (0H)2, is widely used as a sorbent for hydrophobic interaction chromatography. Hydroxyapatite is an underivatized matrix with a specific inherent surface functionality. In the 1980s, it had been introduced as ceramic beaded material with good physical strength (Kato, Nakamura, and Hashimoto, 1987). The interactions of the surface phosphate and caldum-ions with the amino and carboxyl groups of proteins are complex and not fully understood. The elution of proteins is typically... [Pg.52]

Starting from the fact that hexacoordinated ruthenium salts of the general formula [Ru(bpy)2L2]X2 or [Ru(phen)2L2]X2 are capable of forming specific interactions with double stranded DNA, Strekas suggested that the two (A) and (A) enantiomers could be separated by using column chromatography with double-stranded DNA immobilized on hydroxyapatite as the stationary phase. [Pg.57]

Hydroxyapatite chromatography Single- and double-stranded DNA Hydroxyapatite solid surface Single- and double-stranded DNA have different interaction affinities with hydroxyapatite... [Pg.3431]


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Hydroxyapatite

Hydroxyapatite chromatography

Hydroxyapatites

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