Chromatography forms

O. Nuyken, and R. Weidner, Advanced Polymer Science, Chromatographies-Forms-Copolymers, (Ed. H. J. Can-tow), Springer Verlag, Berlin, Vol. 73-74, p. 145 (1986). 

The isolation and cleanup of biological macromolecules by means of affinity chromatography form another typical example of specific application of sample treatment. Affinity chromatography exploits specific functional properties of molecules as retardation of specific groups of solutes in the adsorption step takes place. Later on, adsorbed... 

Maniska A and Komysova O. Continuous beds (monoliths) Stationary phases for liquid chromatography formed using the hydrophobic interaction-based phase separation mechanism. J. Biochem. Biophys. Methods 2004 59 1 8. 

Multizonal development the use of multicomponent eluents which are partially separated during development (frontal chromatography), forming an eluent strength gradient along the layer... 

Thus the first level of compositional information is group type totals as deduced by adsorption chromatography form the distribution of saturates, olefins, and aromatics in materials that boil below 315°C (600°F) (ASTM D-1319). Adsorption methods (ASTM D-2007) can also be used to determine hydrocarbon types in kerosene, but, with all adsorption methods, allowances must be made for the loss of volatile constituents during the workup procedure. Thus column chromatography would be best done with a stabilized (volatile constituents removed to a predetermined temperature) feedstock. 

Figure 21.16 shows a typical anion analysis by ion chromatography. Such analyses would be difficult to perform by other methods. Ion chromatography forms the basis of automatic amino acid analysis. 

As the migration time of the micellar phase, tmc, approaches infinity, i.e. the micellar phase becomes stationary, the expression for k resumes the conventional chromatography form (equation 2.1). 

Smith et al. (1971) have determined the amount of polymers (i.e., substances which elute in the higher molecular weight range on Sephadex G-25 gel chromatography) formed in aqueous solutions (25% W/V) of a number of penicillins stored at room temperature. The solutions were not buffered. The results obtained are shown in Table 1. In another study, these investigators (Dewdney et al. 1971) have demonstrated that the amounts of polymers formed in a 25% W/V solution of benzylpenicillin sodium are greatly reduced if a phosphate buffer (pH 7.5) is included in the solution. A similar observation has also been made by de Weck et al. (1968) in the case of a 10% W/V penicillin solution. This reduction in polymerization... 

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