Chromatography exchange... reversed-phase

Figure 4.27 Flow chart for coluwi selection based on sample type (m - molecular weight). PLC precipitation-liquid chromatography SEC = size-exclusion chromatography lEC - ion-exchange chromatography HIC hydrophobic interaction chromatography LSC liquid-solid chromatography RPC - reversed-phase liquid chromatography BPC (polar) bonded-phase chromatography and IPC - ion-pair chromatography.

Tjemberg, A., Edlund, P. O., and Noren, B., Screening of eltanolone metbolites in dog urine by anion-exchange/reversed phase liquid chromatography and mass spectrometry, /. Chromatogr. B, 715, 395, 1998. [Pg.313]

The TLC process is an off-line process. A number of samples are chromatographed simultaneously, side-by-side. HPTLC is fast (5 min), allows simultaneous separation and can be carried out with the same carrier materials as HPLC. Silica gel and chemically bonded silica gel sorbents are used predominantly in HPTLC other stationary phases are cellulose-based [393]. Separation mechanisms are either NPC (normal-phase chromatography), RPC (reversed-phase chromatography) or IEC (ion-exchange chromatography). RPC on hydrophobic layers is not as widely used in TLC as it is in column chromatography. The resolution capabilities of TLC using silica gel absorbent as compared to C S reversed-phase absorbent have been compared for 18 commercially available plasticisers, and 52 amine and 36 phenolic AOs [394]. [Pg.221]

Stepnowski, P. and Mrozik, W., Analysis of selected ionic liquid cations by ion exchange chromatography and reversed-phase high performance liquid chromatography, /. Sep. Sci, 28,149-154, 2005. [Pg.179]

A. J. Szuna, T. E. Mulligan, B. A. Mico and R. W. Blain, Determination of Ro 23-7637 in dog plasma by multidimensional ion-exchange-reversed-phase high-performance liquid chromatography with ultraviolet detection , J. Chromatogr. 616 297-303 (1993). [Pg.292]

Liquid chromatographic analyses with ICP-MS detection may be divided into categories according to the mode of chromatography used reversed-phase, ion-pair, micellar, ion-exchange, and size-exclusion chromatography. [Pg.381]

Self-displacement will operate with almost any mode of chromatography including reversed phase as demonstrated above, normal phase, chiral and ion-exchange. The following procedures provide a generic approach to development of a... [Pg.86]

Abbreviations for type of application IEX Ion Exchange, HIC Hydrophobic interaction chromatography, AF Affinity chromatography, RP Reverse phase, IMAC Immobilized metal affinity chromatography. [Pg.455]

The resulting active fraction was further processed in five steps of high performance liquid chromatography (HPLC) reverse phase HPLC on Develosil 5Co (Nomura Kagaku) with 20-25% acetonitrile in 0.08% trifluoroacetic acid (TEA) (step 12), reverse phase HPLC on Hi-Pore RP-304 (Bio-Rad) with 20-40% acetonitrile in 0.08% TEA (step 13), reverse phase HPLC on Hi-Pore RP-304 with 20-40% acetonitrile in 0.1% heptafluorobutyric acid (HEBA), cation exchange HPLC on TSKgel (Toyo Soda) with... [Pg.21]

Vergara, C. Mardones, C. Hermosin-Gutierrez, 1. von Baer, D. 2010. Comparison of high-performance liquid chromatography separation of red wine anthocyanins on a mixed-mode ion-exchange reversed-phase and on a reversed-phase column. J. Chromatogr. A. 1217 5710-5717. [Pg.179]

Note lEX = ion-exchange chromatography AF = affinity chromatography IMAC = (metal-)chelate chromatography RP = reversed-phase chromatography. ... [Pg.51]

Affinity chromatography Ion-exchange chromatography (Metal-)chelate chromatography Size-exclusion chromatography Normal/reversed-phase chromatography... [Pg.53]

So far HPLC analysis of alkaloids has been performed by means of ion-exchange, reversed-phase, ion-pair and straight-phase chromatography. Also, some general aspects of the alkaloid analysis by means of these types of chromatography will be dealt with in this chapter. [Pg.223]

The analytical methods used must be reproducibly validatable to ensure regulatory compliance for the FDA and confidence in the quality of a potentially successful, marketable product. Some of the most often used analytical methods are the chromatographic techniques such as size exclusion, ion exchange, affinity chromatography, and reverse-phase chromatography. [Pg.328]

The most commonly used chromatography matrices are affinity, ion-exchange, reverse-phase, and molecular sieving matrices. An affinity matrix contains a specific ligand that binds only the protein of interesta specific enzyme inhibitor or a particular DNA sequence can be used this way. The protein of interest binds to the column and the other proteins in the mixture pass through. The protein is then eluted with a solvent that breaks the interaction between the ligand and the protein so that the protein can be eluted in pure form. [Pg.119]

The formation of a highly reactive nitrene can be used for immobilization of a polymer. The in-situ crosslinking of polymers is induced by exposure to UV light (350 nm) of polymers prepared by free-radical polymerization of the desired monomer to which a pyridinium ylid-type monomer is added in small amounts. Depending on the final product requirements, more than one monomer can be incorporated. This flexibility allows one to tailor a process in a very simple manner. The immobilization of reactive polymer on various surfaces produces a template from which numbers of separation can be performed ion exchange, reverse phase, affinity, or chiral chromatography. The support can also be used in product preparation such as DNA/peptide synthesis. [Pg.94]

Abbott, T, Peterson, R., McAlpine, J., Tjarks, L., and Bagby, M. (1989) Comparing centrifugal countercurrent chromatography, nonaqueous reversed phase HPLC and Ag ion exchange HPLC for the separation and characterization of triterpene acetates. J. Liquid Chromatogr. 12, 2281-2301. [Pg.260]

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