Chondroitin sulfate, carbohydrate-protein linkage

The carbohydrate-protein linkages of chondroitin sulfate (CS) and dermatan sulfate (DS) in collagen-associated PGs, have10,11 the general struc-... 

Oligosaccharide Fragments Isolated from the Carbohydrate-Protein Linkage-Region of Chondroitin Sulfate Proteoglycans... 

Sugahara K, Yamashina 1, Dewaard P, Vanhalbeek H, Vhegen-thart JFG. Structural studies on sulfated glycopeptides from the carbohydrate-protein linkage region of chondroitin 4-sulfate proteoglycans of swarm rat chondrosarcoma - demonstration of 86. the structure Gal(4-0-Sulfate)-P-l-3-Gal-P-l-4Xyl-P-l-0-Ser. J. 

H19. Helting, T., and Rod6n, L., Biosynthesis of chondroitin sulfate. I. Galactosyl transfer in the formation of the carbohydrate-protein linkage region. J. Bid. Chem. 244, 2790-2798 (1969). 

A number of other problems pertaining to the biosynthesis of dermatan sulfate could be mentioned, but since this entire area is still a matter of speculation rather than actual knowledge, only one additional question will be pointed out. It has been indicated by preliminary experiments that at least the immediate sequence of amino acids surrounding the carbohydrate-protein linkage in dermatan sulfate from skin is different from that found in the chondroitiii sulfate-protein of bovine nasal cartilage (Stern and Rod6n, 1969). It wall therefore be of interest to study the acceptor specificity of the xylose transfer reaction, since tw o different xylcffiyltransferases could conceivably be involved in the formation of dermatan sulfate and chondroitin sulfate. 

The O-glycoside linkage between protein and carbohydrate exists in blood group substances and chondroitin sulfate. The others are N-asparaginyl linked. 

Fio. 4. structure of the carbohydrate sequence of the chondroitin sulfate-protein linkage region (the sulfate group on the galactosamine residue is omitted). 

Figure 2. Chondroitin sulfate chains covalently attach to a core protein via a carbohydrate linkage region. The native chondroitin sulfate epitopes for various anti-chondroitin sulfate monoclonal antibodies have been mapped to different regions of the chondroitin sulfate chains of embryonic chick aggrecan. The native 3B3 epitope [12, 48] appears at the non-reducing terminus. Interior domains contain epitopes for antibodies 4D3I6C3/CS-56 [62]. Epitopes for antibodies 4C3j7D4 are located adjacent to the linkage region [25, 62]. Figure modified from [62].

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