Chlortetracycline , fluorescent

DasGupta, S., Mills, C.L., and Fraser, L.R. (1993). Ca -related changes in the capacitation state of human spermatozoa assessed by a chlortetracycline fluorescence assay. J. Reprod. Fertil. 99 135-143. 

Fraser, L.R. and Herod, J,E. (1990). Expression of capacitation-dependent changes in chlortetracycline fluorescence patterns in mouse spermatozoa requires a suitable glycolysable substrate. J, Reprod. Fertil. ( 8 611-621. 

Lee, M.A., Trucco, G.S., Bechtol, K.B., Wummer, N., Kopf, G.S., Blasco, L and Storey, B.T. (1987). Capacitation and acrosome reactions in human spermatozoa monitored by a chlortetracycline fluorescence assay. Fert. Steril. 48 649-658. 

Ward, C.R. and Storey, B.T. (1984). Determination of the time course of capacitation in mouse spermatozoa using a chlortetracycline fluorescence assay. Dev. Biol. 704 287-296. 

Owen, N.E. and LeBreton, G.C. (1981). Ca mobilization in blood platelets as visualized by chlortetracycline fluorescence. Am. ]. Physiol, 241, H6I3-H619... 

An important area of direct biochemical interference is that caused by fluorescent or fluorescent quenching materials in the blood or urine after the administration of a drug. These interferences may be observed during catecholamine analysis in urine from patients receiving -methyldopa, tetracyclines, chlortetracyclines, oxytetracycline, erythromycin, chlorpro-mazine, or quinidine (A2, G5). 

The incidence of tetracycline residues in turkeys has been also studied recently (13). During meat inspection, suspicious flocks were identified by fluorescence detection in bones. A total of 85 flocks showed significant fluorescence in bones. When bones, liver, kidney, and muscle of these animals were analyzed for tetracyclines, residues of tetracycline, chlortetracycline, oxytetracycline, or doxycycline were found in 83 flocks. These results suggest that the detection of fluorescence in bones might be a useful screening method for tetracycline residues in poultry carcasses. 

The reduction in the fluorescence of chlortetracycline which occurs when it binds Ca has also been used to show that changes in the intracellular concentration of Ca occur when the formation of Of is stimulated in PMNs. When PMNs which had been treated with chortetracycline were stimulated with either cytochalasin D or E. Coli, a decrease in fluorescence occurred which followed closely the release of O2 The antagonist of Ca , TMB8 inhibited both the change in fluorescence and the formation of O2" by PMNs, leading the authors to conclude that translocation of Ca was required for the activation of PMNs by these two stimuli. 

RJ McCracken, W John, WJ Blanchflower, SA Haggan, DG Kennedy. Simultaneous determination of oxytetracycline, tetracycline and chlortetracycline in animal tissues using liquid chromatography, postcolumn derivatization with aluminum, and fluorescence detection. Analyst 120 1763-1766, 1995. 

Spectrofluorimetry differs from absorption spectrophotometry in not yielding an absolute scale of values. For this reason it is essential to employ a reference standard for quantitative measurements. For example, some pharmacopoeial tests, such as the test for uniformity of content for digitoxin tablets, employ a spectrofluorimefric assay and comparison wi an ofticial reference standard. Quantitative Spectrofluorimetry has been proposed for a munber of naturally fluorescent compoimds, including ergometrine, riboflavine, tiie catechol-amines, phenothiazines, the barbiturates (at pH 13), and certain antibiotics such as chlortetracycline and oxytetracycline. 

Naidong, W., Hua, S., Roets, E., Hoogmartens, J. Assay and purity control of tetracycline, chlortetracycline and oxytetracycline in animal feeds and premixes by TLC densitometry with fluorescence detection. J. Pharm. Biomed. Anal. 33, 85-93 (2003)... 

Saling, P.M. and Storey, B.T. (1979). Mouse gamete interactions during fertilization in vitro Chlortetracycline as fluorescent probe for the mouse sperm acrosome reaction. J. Cell Biol. 83 544-555. 

In order to pursue this question we developed a technique which permitted the measurement of intracellular Ca + movements in intact platelets (22). This was accomplished through the use of a Ca +-sensitive fluorescent probe, chlortetracycline (CTC), and a photon counting microspectro-fluorometer. 

A Hber-optic sensor for calcium ions has also been proposed recently [85]. It is based on the fluorescent chelate of chlortetracycline (CTC) formed in aqueous solution at pH 7.5. The... 

Molecular inclusion was demonstrated by the aqueous solubilization and UV and fluorescence analysis of the guest dyes phenol blue (8), pinacyanol chloride (9). chlortetracycline (10).and naphthalene (11).Fluorescence lifetime decay experiments using 1,6-diphenylhexatriene (12) as the probe further supported the host-guest relationship while electron microscopy (EM) coirobo-rated the single molecule, non-aggregated state. Calculated molecular modeling diameters o/48 A in a fully extended conformation were also substantiated via EM. 

Although the application of CE-LIF to this analytical field is still limited, there are some examples, such as the determination of tetracycline, oxytetra-cycline, and chlortetracycline in milk samples based on the formation of the corresponding fluorescent chelates with magnesium ions. 

Although highly fluorescent, tetracycline antibiotics also have several electroactive moieties. The EC behavior of tetracycline, oxytetracycline, chlortetracycline... 

Expose chromatogram to ammonia vapor and view under UV light. Most compounds give yellow to orange spot iso compounds, blue fluorescence chlortetracycline, blue fluorescence in solvent A... 

---