Chitinasic

Jolles P, Muzzarelli RAA (eds) (1999) Chitin and Chitinases. Birkhauser, Basel... 

Muzzarelli RAA, Mattioli-Belmonte M, Pugnaloni A, Biagini G (1999) In Jolles P, Muzzarelli RAA (eds.) Chitin and Chitinases. Birkhauser, Basel, p. 251... 

Chitinases are responsible for the breakdo vn and recycling of chitin. With several gigatons of chitin being produced annually, it is the second most abundant polymer... 

Metraux, J.P. Boiler, Th. (1986). Local and systemic induction of chitinase in cucumber plants in response to viral, bacterial and fungal infections. Physiological and Molecular Plant Pathology, 28, 161-9. 

Sanchez-Monge R, Dlaz-Perales A. Blanco C. Salcedo G Latex allergy and plant chitinases in Mills ENC, Shreffler WG (eds) Plant Food Allergens. Oxford. Blackwell Science. 2004, pp 87-104. 70... 

Blanco, C., Diaz-Perales, A., Collada, C., Smchez-Monge, R., Aragoncillo, C., Castillo, R., Ortega, N., Alvarez, M., Carrillo, T., and Salcedo, G. (1999). Class 1 chitinases as potential panallergens involved in the latex-fruit syndrome. /. Allergy Clin. Immunol. 103, 507-513. 

IV. PARASITISM. Lysis by hydrolytic enzymes excreted by microorganisms is a well-known feature of mycoparasitism. Chitinase and P-l,3 glucanase (laminarase) are particularly important enzymes secreted by fungal mycoparasites capable of degrading the fungal cell wall components, chitin, and P-1,3 glucan (131-134). 

Many rhizobacteria are classified as chitinolytic and, for example, Serratki marsescens, which excretes chitinase, was found to be an effective biocontrol agent against Sclerotium rolfsii (135). Similarly, Aeromonas caviae was found to reduce disease caused by Rhizoctonia solani, Fusarium oxysporuin, and Sclerotium rolfsii (136). There is also evidence to support the role of P-l,3 glucanase in biocontrol of soil-borne plant pathogens (137). 

J. Inbar and I. Chet, Evidence that chitinase produced by Aeromonas caviae is, involved in the biological control of soil-borne plant pathogens by this bacteria. Sod Biology and Biochemistry 23 913 (1991). 

Hakala BE, White C, Recklies AD 1993 Human cartilage gp-39, a major secretory product of articular chondrocytes and synovial cells, is a mammalian member of a chitinase protein family. J Biol Chem 268 25803-25810... 

Hu B, Trinh K, Figueira WF, Price PA 1996 Isolation and sequence of a novel human chondrocyte protein related to mammalian members of the chitinase protein family. J Biol Chem 271 19415-19420... 

Watanabe T, Kobori K, Miyashita K et al 1993 Identification of glutamic acid 204 and aspartic acid 200 in chitinase Al of bacillus circulans WL-12 as essential residues for chitinase activity. J Biol Chem 268 18567-18572... 

Assenga, S.P, You, M., Shy, C.H. et al. (2006) The use of a recombinant baculovirus expressing a chitinase from the hard tick Haemaphysalis longicornis and its potential application as a bioacaricide for tick control. Parasitology Research, 98 (2), 111-118. 

Fig. 10.1. Localization of Acanthocheilonema viteae chitinase in intrauterine and newborn mf by IFAT with mAb 24-4 (left panels) and corresponding light microscope photographs (right panels). (A), (B) Uterine contents of female A. viteae with nearly mature mf inside the eggshell, younger embryonic stages and a mature, hatched mf. (C), (D) Newborn mf. Note that fluorescent mf are swollen and stumpy. Bars represent 50 pm es, embryonic stages nmf, nearly mature mf hmf, hatched mf.

Fig. 10.2. Immunogold staining of an ultra-thin section of an immature Acanthocheilonema viteae uterine mf with mAb 24-4. Note that A. viteae chitinase is present in the cuticle (arrowheads), but not on the surface. (Photograph W. Rudin.)...

---