DNA chimeric

The first biologically functional chimeric DNA molecules constructed in vitro were assembled from parts of different plasmids in 1973 by Stanley Cohen, Annie Chang, Herbert Boyer, and Robert Helling. These plasmids were used to transform recipient E. coli cells transformation means the uptake and repli-... 

Restriction Enzymes DNA Ligase Are Used to Prepare Chimeric DNA Moiecuies... 

Chimeric DNA molecules are introduced into cells to make transfected cells or into the fertilized oocyte to make transgenic animals. 

Finally, the chimeric DNA is reinserted into a host cell, such as a bacterium. If the procedure is carried out properly, the chimeric DNA begins to function just as it would in any bacterial cell. The hacterial genes it contains continue to function as they normally... 

Chimeric DNA. Recombinant DNA the components of which originate from two or more different sources. 

Frimerman, A., Welch, P.J., Jin, X., Eigler, N., Yei, S., Forrester, J. et al. (1999) Chimeric DNA-RNA hammerhead ribozyme to proliferating cell nuclear antigen reduces stentinduced stenosis in a porcine coronary model. Circulation, 99, 697-703. 

Taylor, N.R., Kaplan, B.E., Swiderski, P., Li, H. and Rossi, J.J. (1992) Chimeric DNA-RNA hammerhead ribozymes have enhanced in vitro catalytic efficiency and increased stability in vivo. Nucleic Acids Res., 20,4559—4565. 

Multiple efforts have been made to replace phosphorous-containing linkages with sulfur-containing isosteres in the context of enzyme inhibition. In a search for nonionic transition state analog inhibitors of restriction enzymes, Blattler et al. (34) found that nucleic acid duplexes that incorporate a dimethyl sulfone in place of a phosphodiester have distorted backbones similar to those in restriction enzyme bound DNA. Chimeric DNAs that incorporate sulfone hnkages were synthesized, and depending on the location of the dimethylene sulfone hnker, either between the first AT unit or the second AT unit in the EcoRV recognition site, values were 20 nM and 120... 

There are two reports describing the use of nucleosides with other sugar conformations. A method for preparing internally P-labelled l-DNA has been described using T4 polynucleotide kinase. Chimeric DNA composed of tandem a- and p-anomeric strands have been used in TFOs and shown to have enhanced thermal stability compared to all a- or p-anomeric oligonucleotides. ... 

Restriction enzymes, which cleave within short, specific sequences of DNA, can be used to obtain DNA fragments for study or for insertion into DNA from other sources. The fusion product is known as chimeric DNA. 

Restriction fragments of DNA can be used to identify variations in base sequence in a gene. However, they also can be used to synthesize a recombinant DNA (also called chimeric DNA), which is composed of molecules of DNA from different... 

DNA molecules containing covalently linked segments derived from two or more DNA sources are called recombinant DNA. (Another name for recombinant DNA is chimeric DNA, named after the chimera, a monster in Greek mythology that had the head of a lion, the body of a goat, and the tail of a serpent.) The production of recombinant DNA was made possible by the isolation of restriction endonucleases. 

Inoue, H, Hayase, Y, Iwai, S, and Ohtsuka, E. (1988) Sequence-specific cleavage of RNA using chimeric DNA splints and RNase H. Nucl Acids Res. Symposium Series No. 19, 135-138. 

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