Chemical intramolecular inclusion complexe

Intramolecular [2 + 2] photocyclization reactions of 2- /V-(2-propcnyl)amino]cy-clohex-2-enones (67) are also controlled enantioselectively by carrying out irradiation in inclusion complex with a chiral host compound. When inclusion crystals of 67 with 12 are irradiated in the solid state, optically active 9-azatricyclo[5.2.1.0]-decan-2-ones (68) were obtained in the chemical and optical yields indicated in Table 3 [30],... 

The analysis of heterologous proteins in recombinant hosts, such as E. coll, presents many challenges to the analytical biochemist. The cells must be lysed and the inclusion bodies solubilized prior to quantification. Cell lysis and protein solubilization can be accomplished chemically through the use of SDS. As the proteins in inclusion bodies can exist as a distribution of forms, such as covalent and noncovalent polymers, it is crucial to convert the target protein into a single molecular entity prior to analysis. This can be achieved by unfolding the proteins and disrupting the inter- and intramolecular disulfide bonds via reduction or sulfitolysis. The complexity of the matrix adds to the difficulty in the determination of the recombinant protein, as both the whole cell and the inclusion bodies can also contain nucleic acids, salts, lipids, and other host molecules, in addition to proteinaceous material. 

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