Chelating agents sodium citrate

The mixed lanthanide (III) ions (Ln3+) can be absorbed from solution at the top of a column of a cation-exchange resin RSOsNa (Section 14.4) and then selectively eluted from it, that is, swept down the column in bands by a stream of a solution of a substance that competes with the Ln3+ for sites in the resin. An acidic tribasic chelating agent H3X (usually citric acid/sodium citrate buffer) is used so that, as in reaction 17.16, the tendency for a specific Ln3+ to form neutral LnX and so escape the electrostatic... 

Avoidance of interference of other milk constituents with measurements is also of importance for example, dissociation of casein micelles by calcium-chelating agents, such as trisodium citrate or ethylenediamine tetra-acetic acid (EDTA), may used to avoid interference of the micelles in particle size measurement, while clusters of fat globules can be disrupted by adding a low level of sodium dodecyl sulphate (SDS). 

The isolation of bacterial DNA described in this experiment, patterned after the work of Marmur (1961), accomplishes these objectives. Bacterial cells are disrupted by initial treatment with the enzyme, egg-white lysozyme, which hydrolyzes the peptidoglycan that makes up the structural skeleton of the bacterial cell wall. The resultant cell walls are unable to withstand osmotic shock. Thus, the bacteria lyse in the hypotonic environment. The detergent, sodium dodecyl sulfate, (SDS, sodium do-decyl sulfate) then completes lysis by disrupting residual bacterial membranes. SDS also reduces harmful enzymatic activities (nucleases) by its ability to denature proteins. The chelating agents, citrate and EDTA (ethylenediamine tetraacetic acid), also inhibit nucleases by removing divalent cations required for nuclease activity. 

There have been a number of developments in the APDC—MIBK extraction system. In one of them Kinrade and Van Loon [9] used two chelating agents, APDC and DDDC. They maintained that the DDDC has a stabilising effect on all the metal complexes in the system. In this method, they adjusted the pH of 200 ml of aqueous sample with 4 ml of citrate buffer (1.2M sodium citrate and 0.7 M citric acid) to around pH 5.0. 5 ml of the chelating solution (1% (m/v) each of APDC and DDDC in water) and 35 ml (or less) of MIBK are added and the extraction is carried out as described earlier. By this... 

Sodium citrate/ Citric acid Airet, Isuprel, Tornalate Buffering agent Chelating agent Flavoring agent 0.6%/0.44%... 

The limited success with sodium citrate led to the trial and error testing of other chelating agents. Despite the fact that hard Lewis acids such as plutonium do not bind strongly to sulfur ligands, the success of 2,3-dimercapto-l-propanol, BAL, as an efficient chelator for arsenic79) led to testing its ability to remove actinides. As expected on a chemical basis, excretion of plutonium was not enhanced by treatment... 

Complexing chelating) agents citric acid, tartaric acid, B sodium tartrate), kerosene, segesterene, sodium citrate, oxalic acid, sodium oxalate, or orthophosphoric acid X... 

Sodium tolyltriazole Stearyl citrate Tolyltriazole Trisodium NTA monohydrate chelating agent mfg. 

A number of natural substances, including sodium citrate and ascorbic acid, chelate Pb. Commonly used chelating agents include calcium disodium edetate (CaEDTA), dimercaprol (BAL), 2,2-dimethylcysteine (D-peniciUamine) and 2,3-dimercaptosuc-cinic acid (DMS). Chisholm and Coffin et al." have outlined the appropriate protocols for chelation therapy for children wdth Pb poisoning, whereas Friedheim et al. and the Center for Disease Control, outline the treatment of adult Pb intoxication. 

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