Cesium chloride density

Purified by one or more of the following procedures (i) Cesium chloride density gradient,... 

Representative Algal Taxa from Which DNA Has Been Retrieved Using Fluorochrome-Cesium Chloride Density Gradient Methodologies... 

DNA samples purified by either stringent methods (equilibrium centrifugation in cesium chloride density gradients) or simple methods (organic extraction) have yielded reproducible RAPD patterns. However, in plants (e.g., members of the evening primrose family, Onagraceae) and... 

Figure 3.A.. A, Profile of mouse liver DNA in a preparative Ci O Ag density gradient. (From Thiery el al., 1976), l(. Analylieal cesium chloride density gradient protiles of fractions from mouse liver DNA. Gaussian curves (broken lines) rcprc-scm the different DNA components as resolved using a Dupont 310 curve resolver and the criteria given in the original paper (From Macaya ci al.. 1976).

We have used cesium chloride density gradient centrifugation to clean up phaiol-chloroform extracted, ethanol precipitated DNA and measured the "hyperchromic shift" when dsDNA denatures to ssDNA as an indication of DNA purity. A "hyperchromic shift" of more than 40% indicates that the DNA is of sufficient purity for quantitative hybridization. 

Cesium chloride density gradients show identity between transducing particles and phage at a density of 1.528. 

Estimate the density of each of the following solids from the atomic radii of the ions given in Fig. 1.48 (a) calcium oxide (rock-salt structure, Fig. 5.39) (b) cesium bromide (cesium chloride structure, Fig. 5.41). 

Are the following statements true or false (a) Because cesium chloride has chloride ions at the corners of the unit cell and a cesium ion at the center of the unit cell, it is classified as having a body-centered unit cell, (b) The density of the unit cell must be the same as the density of the bulk material, (c) When x-rays are passed through a single crystal of a compound, the x-ray beam will be diffracted because it interacts with the electrons in the atoms of the crystal, (d) All the angles of a unit cell must be equal to 90°. 

The density of cesium chloride is 3.988 g-em .Calculate the percentage of empty space in a cesium chloride lattice with the ions treated as hard spheres. 

Isolation of Plasmid Enriched DNA. The three strains of B. megaterium were cultured in yeast-soy broth (4% Bacto-yeast extract and 1.6% Bacto-soytone from Difco Laboratories, Detroit, pH 7.2). Plasmid DNA from each strain was isolated and purified by cesium chloride-ethidium bromide equilibrium density centrifugation following the procedure of Carlton and Brown ( ). Subsequent gel electrophoresis (0.5% agarose) revealed the presence of some chromosomal DNA contamination. 

At the top right, the diagram shows the densities and sedimentation coef cients for biomolecules, cell organelles, and viruses. Proteins and protein-rich structures have densities of around 1.3 g cm , while nucleic acids show densities of up to 2 g cm . Equilibrium sedimentation of nucleic acids therefore requires high-density media—e.g., concentrated solutions of cesium chloride (CsCl). To allow comparison of S values measured in different media, they are usually corrected to values for water at 20 °C ( S20W ) ... 

After the run, unload the tube either by puncture of the thin-walled tube with a syringe needle at the bottom, or by displacement by 80% cesium chloride (w/w). For DNA content, read UV absorption of the fractions and refractive index for density calculation (for equations see footnote to Table 5.4). 

What is their volume If the mass of a bacterial ribosome is 2.7 x 106 daltons, what is its apparent density (divide mass by volume) Experimentally the buoyant density of bacterial ribosomes in a cesium chloride gradient (Chapter 5) is about 1.6 g/crn3. How can this difference be explained If eukaryotic ribosomes are 1.17 times larger than bacterial ribosomes in linear dimensions, what is the volume of a eukaryotic ribosome ... 

Template DNA for the production of hybridization probes must be pure Standard methods, such as dye-buoyant density ultracentnfugation, generate acceptable products. Ethidium bromide and cesium chloride are removed prior to use of the DNA (4)... 

Estimate the density of cesium chloride from its crystal structure. 

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