Cerebrospinal fluid pyruvic acid

Ruggeri M, Merlo Rich E, Zini I, Fuxe K, Ungerstedt U, et al. 1990. Indole-pyruvic acid increases 5-hydroxyindoleacetic acid levels in the cerebrospinal fluid and frontoparietal cortex of the rat a microdialysis study. Acta Physiol Scand 138(1) 97-98. 

Lactate and can be measured in blood, plasma, urine and the cerebrospinal fluid (CSF). Pyruvate and ACAC are highly unstable, so deproteinisation is performed immediately after sampling. Lactate, pyruvate, and ACAC determination can be measured in the same sample after deproteinisation using perchloric acid [17]. 

M2. McArdle, B., The quantitative estimation of pyruvic and a-oxoglutaric acids by paper chromatography in blood, urine and cerebrospinal fluid. Biochem. J. 66, 144 (1957). 

The most widely known metabolic disorders are those which result in impairment of the intermediary metabolism of nutrients such as proteins, carbohydrates and lipids. For example, phenylketonuria is due to a genetic deficiency of phenylalanine hydroxylase, an enzyme involved in the conversion of phenylalanine to tyrosine. As a result, when ingested in amounts normally encountered in the diet, phenylalanine accumulates in blood and cerebrospinal fluid along with its pyruvate, lactate and acetate derivatives. (See review by McBean and Stephenson. ) The toxic response takes the form of severe mental retardation, neural and dermal lesions and premature death. But phenylalanine is an essential dietary amino acid and cannot be rigorously excluded from the diet, even of sufferers from phenylketonuria, though fortunately they do respond to reduced dietary intakes. Clearly, phenylalanine hydroxylase deficiency narrows the gap between the required intake and that which elicits a toxic response because this pathway is more readily overloaded . 

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