Cerebrospinal fluid microdialysis

Ruggeri M, Merlo Rich E, Zini I, Fuxe K, Ungerstedt U, et al. 1990. Indole-pyruvic acid increases 5-hydroxyindoleacetic acid levels in the cerebrospinal fluid and frontoparietal cortex of the rat a microdialysis study. Acta Physiol Scand 138(1) 97-98. 

Wang Y, Wong SL, Sawchuk RJ. Microdialysis calibration using retrodialysis and zero-net flux application to a study of the distribution of zidovudine to rabbit cerebrospinal fluid and thalamus. Pharmaceutical Research 1993, 10, 1411-1419. 

Cerebrospinal Fluid (CSF) Microdialysis allows sampling of CSF (Golden et al., 1993 Malhotra et al., 1994 Togashi et al., 1994) without removal of the fluid and without altering intracranial pressure. CSF can be sampled from the lateral ventricles (Becker et al., 1988) or from the lumbal spinal cord area (Marsala et al., 1995), but most often CFS from the cisterna magna is tested (Knuckey et al., 1991 Matos et al., 1992). To date all studies have been performed in the rat. 

Becker JB, Adams F, Robinson TE (1988) Intraventricular microdialysis A new method for determining monoamine metabolite concentrations in the cerebrospinal fluid of freely moving rats. J Neurosci Methods 24 259-269. 

Golden PL, Brouwer KR, Pollack GM (1993) Assessment of valproic acid serum cerebrospinal fluid transport by microdialysis. Phar Res 10 1765-1771. 

Knuckey NW, Fowler AG, Johanson CE, Nashold JR, Epstein MH (1991) Cisterna magna microdialysis of 22Na to evaluate ion transport and cerebrospinal fluid dynamics. J Neurosurg 74 965-971. 

Togashi H, Matsumoto M, Yoshioka M, Hirokami M, Tochihara M, Saito H (1994) Acetylcholine measurement of cerebrospinal fluid by in vivo microdialysis in freely moving rats. Jpn J Pharmacol 66 7-74. 

Wong SL, Van Belle K, Sawchuk RJ (1993) Distributional transport kinetics of zidovudine between plasma and brain extracellular fluid/cerebrospinal fluid in the rabbit— Investigation of the inhibitory effect of probenecid utilizing microdialysis. J Pharmacol Exp Ther 264 899-909. 

Another means of sampling is push-pull perfusion (79) using a probe with discrete inlet and outlet tubes. With this method, a small amount of cerebrospinal fluid is pulled directly from the brain through the outlet tube and replaced with artificial cerebrospinal fluid administered via the inlet tube. This approach has greater spatial resolution than microdialysis and because cerebrospinal fluid is collected directly, no concern develops about incomplete recovery. 

Granero, L. Santiago, M. Cano, J. Machado, A. Peris, J.-E. Analysis of ceftriaxone and ceftazidime distribution in cerebrospinal fluid of and cerebral extracellular space in awake rats by in vivo microdialysis. Antimicrob-Agents Chemother., 1995, 39, 2728-2731... 

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