Artificial cerebrospinal fluid

The ionic composition of ACSF used by our laboratories is presented in Table 1, along with the values reported for arterial plasma, CSF from 

---

Hippocampal slices (400-500 frm) were quickly prepared from male Wistar rats (8- to 9-weeks-old) and maintained in a chamber at 35 °C, where they were continuously perfused with artificial cerebrospinal fluid as described in our previous paper [11]. A bipolar tungsten electrode was placed in the stratum radiatum to stimulate Schaffer collateral and commissural afferents. The evoked potential was extracellularly recorded from the pyramidal cell layer of the CA1 subfield with a glass capillary microelectrode. A single test stimulation (0.05 msec duration) was applied at intervals of 30 sec. Drugs were delivered by perfusion. To induce potentiation of the evoked potentials, tetanic stimulation was applied at the same intensity through the same stimulating electrode as used for the test stimulation. The magnitude of LTP was evaluated by the population spike amplitude 30 min after tetanic stimulation. 

Male Wistar rats (280—320 g of weight) were deeply anaesthetized with halo-thane and subsequendy decapitated. Brain slices were prepared according to a previously described procedure (Geracitano et al., 2003). Briefly, the brain was rapidly removed and corticostriatal coronal slices (300 /nn) were cut from a tissue block with the use of a vibratome (at 20-25 °C) in artificial cerebrospinal fluid solution (ACSF). 

Another means of sampling is push-pull perfusion (79) using a probe with discrete inlet and outlet tubes. With this method, a small amount of cerebrospinal fluid is pulled directly from the brain through the outlet tube and replaced with artificial cerebrospinal fluid administered via the inlet tube. This approach has greater spatial resolution than microdialysis and because cerebrospinal fluid is collected directly, no concern develops about incomplete recovery. 

Artificial cerebrospinal fluid (aCSF) 126 mM NaCl, 3 mM KCl, 1.5 mM MgCl2, 2.4 mM CaCl2, 1.2 mM NaH2P04, 11 mM glucose, 26 mM NaHCOs. This can be prepared as a stock (lOX) solution and diluted fresh daily as needed. 

Preparation of brain slices is an indispensable procedure for a variety of experiments. The goal of the slice preparation is to obtain a thin piece of brain tissue containing the cells of interest and to maintain the slice in a viable (although artificial) condition that is similar to its in vivo environment. In this chapter we describe procedures that are fundamental for brain slice preparation. Because the hippocampus is the most widely used tissue for brain slices, Its isolation will be used here to illustrate the steps of the procedure. Two slicing methods, using either a vibratome or a tissue chopper, are described. Our discussion of these methods covers the steps from the decapitation of the animal to the storage of slices in artificial cerebrospinal fluid (aCSF). Some of the... 

X concentrated artificial cerebrospinal fluid (5X aCSF) 50 xaM HEPES, 10 mA/MgS04, and 6 mA/NaH2p04, pH 7.4. Adjust the pH with 3A/NaOH or lAf Tris-hydroxy-methyl-amino-methane (sec Note 1). 

Artificial cerebrospinal fluid (aCSF) 3 mM KCl, 150 mM NaCl, 2 mM CaCli, 5 mAf HEPES. Prepare using stock solutions 1, 3, and 4, and adjust pH to 7.4 with l.OA/NaOH (stock solution 5). 

Problems with recovery are avoided when sampling the extracellular brain tissue by means of a push-puU cannula. A push-pull cannula consists of two coaxial assembled hollow needles (cannulae) which are implanted into the brain. Artificial cerebrospinal fluid is infused through the inner cannula and withdrawn through the outer cannula. " ... 

Voss LJ, Sleigh JW (2010) Stability of brain neocortical slice seizure-like activity during low-magnesium exposure measurement and effect of artificial cerebrospinal fluid temperature. J Neurosci Methods 192 214-218... 

Artificial cerebrospinal fluid (ACSF). The composition may vary according to different protocols. Usually, ACSF contains (in mM) 125 NaCl, 25 NaHCOs, 2.5 KCl, 1.25 NaH2P04, 1 MgCl2, 25 glucose, 2 CaCl2. 

In order to further characterize the antagonistic interaction observed in vitro between CsTx and TxVIA, we studied their effects in rat brain in vivo by intracerebroventricular injections. Rats implanted with guide cannulae were injected intracranially with toxins dissolved in artificial cerebrospinal fluid. Behavior and activity of the toxin-injected rats were... 

---