Filtration centrifuges

For cakes of high permeabihty, the capillary drain height may be an insignificant fraction of cake thickness, and film drainage becomes the controlling factor in a centrifugal field (7). Under unsteady-state conditions, equation 18 represents the drainable Liquid left in the cake as a function of the centrifugal filtration parameters ... 

SO that the batch may be dumped into a tank for processing by successive steps, which normally include centrifugation, filtration, and/or drying. In some cases, a small heal of slurry is left in the batch ciys-tallizer to act as seed for the next batch. 

Tneory of Centrifugal Filtration Theoretical prechc tions of the behavior of sohd-hquid mixtures in a filtering centrifuge are more (difficult compared to pressure and gravity filtration. The area of flow and driving force are both proportional to the radius, and the specific... 

Drying usually follows dewatering (thickening, centrifugation, filtration etc.). The purposes of drying can be several, including. 

Grace, H.P., 1953. Resistance and compressibility of filter cakes. Part III Under conditions of centrifugal filtration. Chemical Engineering Progress, 49, 427M36. 

Zeitsch, K., 2000. Centrifugal Filtration. In Solid-liquid separation, 4th edition. Ed. L. Svarovsky. Oxford Butterworth-Heinemann. 

Stir with 1000ml 50mM CDTA at pH 6.5 for 6h at 20-22X. Filter on G3 glass filter, wash residue with water, centrifuge filtrate at SOOOrpm for 20min, dialyse, concentrate. Reextract the residue under the same conditions and freeze-dry the combined filtrates CDTA-Fraction... 

Occasionally, silica gel and bonded-phase material may be leached from the column and be introduced into the purified sample. This may be removed by centrifugation, filtration, or extraction of the sample with nonpolar solvents, such as hexane. 

The DCL was created under standard disulfide exchange conditions at pH 7.5. After equilibration for 48 hours, each of the expected 15 disulfide products could be observed in the library using LC-MS analysis (Fig. 2.11). The library was then equilibrated in the presence of CaM, followed by a centrifugation filtration step to separate protein/bound components from free components in solution. Analysis of the filtrate was complicated by the filtration membrane affecting the composition of the library. The bound components, however, provided meaningful results. Denaturation and filtration afforded a mixture of all peptides that had bound to CaM in the course of the DCL. HPLC analysis indicated significant amplification of dimer cc (80%) and a small amplification of dimer ec (10%). Resynthesis of these two components and binding assay established values of 10 and... 

For cheese sample Ultraturrax homogenization (two cycles) with H2O, centrifugation (for skimming), acidification at pH 4.2. 6 (for casein precipitation), centrifugation, filtration For cheese sample extraction with H2O (sonication) for milk centrifugation, acidification (pH 4.6) to precipitate caseins, centrifugation, filtration... 

Extraction with H2O (poUtron homogenizer), centrifugation, filtration... 

Extraction with 0.01 N HCl, centrifugation, filtration through glass wool Extractio (0.5%)... 

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