Centella asiatica asiaticoside from

Asiaticoside, an ursane-type triterpene saponin from Centella asiatica has been shown to stimulate skin human fibroblasts collagen I synthesis in vitro. By comparison asiaticoside and asiatic acid, it seemed that the sugar moiety was not necessary for this activity [223]. 

Mahato, S.B. Sahu, N.P. An improved process for the isolation of asiaticoside, useful as an antileprotic agent from Centella asiatica, Indian patent filed, 1993. 

Asiaticoside. Saponin-like triterpene glycoside C48H78O, Mr 959.14, mp. 235-238°C, [aln -14° (C2H5OH) from the leaves of the Asian marsh pennywort, Centella asiatica Hydrocotyle asiatica, Apia-ceae). A. shows antibiotic activity against Gram-positive bacteria and mycobacteria and has a wound-healing effect. 

Aerial material from Centella asiatica was extracted and characterized for asiaticoside content [413]. A 70/30 methanol/water (1% TFA) mobile phase and a Cjg column (A = 220 nm) provided separation of the analyte from numerous other compounds. Elution was complete in <10min. A linear range for asiaticoside was reported as 1-30 pg injected. 

Four triterpenes (asiatic acid, madecassic acid, asiaticoside, madecassoside) were extracted from Centella asiatica and baseline resolved on a Cj colunm... 

A modified TLC method from the European Pharmacopoeia was used for identification of four triterpenoids from Centella asiatica [30]. The plant extract was applied onto a silica gel 60 F254 TLC plate, which was developed using ethyl acetate-methanol (60 40, v/v). The bands were visualized by anisaldehyde derivatization reagent. The identity of four determined triterpenoids—madecassoside, asiaticoside, madecassic acid, and asiatic acid— was confirmed by offline TLC-MALDI-MS analysis. The adsorbent containing the analytes was scraped from the TLC support and the triterpenoids were reextracted using methanol and subjected to MALDI plates, along with the matrix, for MS analysis. 

---