Cellulose cyanogen bromide activation

Bartling GJ, Brown HD, Forester LJ, Koes MT, Mather AN, and Stasiw RO. A study of the mechanism of cyanogen bromide activation of cellulose. Biotechnol. Bioeng. 1972 14 1039-1044. 

A specific test to detect serum IgE antibodies to chlorhexidine is now available in the form of an ImmunoCAP (Thermo Scientific) test. The drug-solid phase is prepared by coupling 1-[A -(p-chlorophenyl)biguanido]-6-aminohexane which represents half of the symmetrical bivalent chlorhexidine molecule to cyanogen bromide-activated cellulose sponge. 

C. Oligo- and Poly-nucleotides.—The stepwise enzymatic synthesis of internucleotide bonds has been reviewed. A number of polynucleotides containing modified bases have been synthesised " in the past year from nucleoside triphosphates with the aid of a polymerase enzyme, and the enzymatic synthesis of oligodeoxyribonucleotides using terminal deoxynucleotidyl transferase has been studied. Primer-independent polynucleotide phosphorylase from Micrococcus luteus has been attached to cellulose after the latter has been activated with cyanogen bromide. The preparation of insolubilized enzyme has enabled large quantities of synthetic polynucleotides to be made. The soluble enzyme has been used to prepare various modified polycytidylic acids. ... 

This method was used, for example, for the solid-phase immunoassay of thyroxine (affinity chromatography). Various activation methods (CDI, periodate, and cyanogen bromide procedures) were compared with each other for coupling antibodies to magnetizable cellulose/iron oxide solid-phase particles. 211]... 

Cyanogen bromide (CNBr)-activated insoluble matrices, such as microcrystalline cellulose particles, or paper disks, can be chemically... 

The most widely used activation procedure of agarose, dextran, but less common of cellulose and hydroxyalkyl methacrylate gel, employs cyanogen bromide [95]. The... 

Derivatives of cellulose continue to be used as supports for new affinity chromatography media and for immobilization of enzymes. Trypsin has been immobilized on 4-aminobenzyl-cellulose. A penicillinase from Bacillus cereus has been immobilized by glutaraldehyde-mediated reaction with aminoethyl-cellulose. Tannin has been activated with cyanogen bromide and coupled with... 

Reaction with cellulose fibres activated with cyanogen bromide... 

Hydroxyl Polysaccharides (cellulose, agarose, dextran, chitin, chitosan), inorganic glass, polyvinyl alcohol, polyhydroxyethyl methacrylate Cyanogen bromide, triazine method, cyclic frans-2,3-carbonate reaction, carbonylation, periodate oxidation, epoxide activation [168-181]... 

Kennedy and Paterson [99] used a similar approach and rolled a rod from a paper-like cellulose sheet. After cyanogen bromide or l,l -carbonyldi-imidazole activation, protein A and protein G were immobilized onto the cellulose and the column was successfully used for the affinity separation of immunoglobulin G (IgG). 

Active immobilized forms of fS-D-glucosidase have been prepared by reaction of the enzyme with cellulose cyclic imidocarbonate, glutaraldehyde-treated beads of polyacrylamide, copolymers of acrylamide and 2-hydroxyethyl-methacrylate treated with cyanogen bromide, and copolymers of acrylamide and acrylic acid, and by entrapment of the enzyme in beads of polyacrylamide. 

Hydrophobic interactions with cellulose esters and with phenoxyacetylated-dextran and other derivatives of glass Reaction with acylazide-activated collagen Reaction with cyanogen bromide-treated copolymers of acrylamide and 2-hydroxyethyl methacrylate Reaction with glutaraldehyde-treated polyacrylamide beads... 

Polysaccharides and cross-linked polysaccharides can be activated by reaction with alkaline cyanogen bromide (reaction 7.19). Polysaccharides such as cross-linked dextran, agaran, and cellulose have been activated by this reaction [37,38]. The activated polysaccharides can then react with amino groups of various ligands that can be used in affinity chromatography [39], or they can react with amino groups on the surface of enzymes to give immobilized enzymes [40] (reaction 7.20). 

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