Cellulose column chromatography

Purified by Sephadex G-200 filtration and DEAE-cellulose column chromatography. Hexosaminidase A was further purified by DEAE-cellulose column chromatography, followed by an ECTEOLA-cellulose column, Sephadex-200 filtration, electrofocusing and Sephadex G-200 filtration. Hexosaminidase B was purified by a CM-cellulose column, electrofocusing and Sephadex G-200 filtration. [Srivastava et al. 7 Biol Chem 249 2034 1974.]... 

Purified from blood using CM-32 cellulose column chromatography. [Matsukawa et al. J Am Chem Soc 107 1108 1985.] For the purification of the a and P chains see Hill et al. Biochem Prep 10 55 1963. 

Hoffmann GF, Brendel SU, et al. (1992) Mevalonate kinase assay using DEAE-cellulose column chromatography for first-trimester prenatal diagnosis and complementation analysis in mevalonic aciduria. J Inherit Metab Dis 15 738-746... 

Nagasawa, T., Kiyosawa, I., Kuwahara, K. and Ganguly, N. C. 1973. Fractionation of buffalo milk casein by acrylamide gel electrophoresis and DEAE cellulose column chromatography. J. Dairy Sci. 56, 61-65. 

Ribadeau-Dumas, B., Maubois, J. L., Mocquot, G. and Gamier, J. 1964. A study of casein by DEAE-cellulose column chromatography in urea. Biochim. Biophys. Acta 82, 494-506. 

Fractions H and /. Paper chromatography indicated that these two fractions were identical except for a few minor spots. The combined fractions were purified by cellulose column chromatography with the upper layer of 4 1 5 w-butanol-ethanol-water as the developer. Over 95% of the combined fraction was recovered as pure oxalic acid. 

A mixture of 0.44 g of acidic proteinoid, 0.19 g of basic proteinoid, and 0.31 g of radioactive ATP in 2.0 ml of 0.02 M MgCl2, 0.05 M Tris buffer, is heated in a boiling water bath for 5 min, and then incubated at 37 °C for 24 hrs, after the fractionation of the mixture by DEAE-cellulose column chromatography. Oligonucleotides produced are identified with those of authentic markers 47). Chain length has been determined by the ratio of AMP to adenosine in the alkaline hydrolyzate of the material which was treated by alkaline phosphatase to remove 5 - and 3 -phosphate47). 

Partial-acetolysis studies conducted on yeast D-mannopyranans containing (l- 6)-linked a-D-mannopyranosyl main-chains revealed a large variety of side-chain structures, as represented by oligosaccharides isolated from the acetolyzate (see Table III). Following purification by cellulose-column chromatography, the oligosaccharides were identified by their typical, 13C-n.m.r. spectra.85... 

The key to success in extensive purification and crystallization of ferredoxin is the use of diethylaminoethyl (DEAE) cellulose column chromatography. Ferredoxin has a marked affinity for DEAE-cellulose, and when a cell-free extract containing ferredoxin is passed through a DEAE-cellulose column, ferredoxin remains as a band at the top while contaminating protein passes through in the effluent. The affinity of ferredoxin for DEAE-cellulose may be explained by its isoelectric point of 3.7 (Lovenberg, Buchanan, and Rabinowitz (65)). 

Sugars, Analysis of Mixtures, by Paper and Cellulose Column Chromatography... 

The authors found that over 15 different aseptically prepared lysine-rich proteinoids 18 Table XII) each had about the same level of activity, that the pH optimum was near 7, and that Michaelis-Menten kinetics were obeyed. CMC (carboxymethyl cellulose) column chromatography of one polymer yielded nine fractions, four of which showed activity. Control experiments lacking one or more of polymer, Cu2+, urea, or KGA did not produce detectable amounts of glutamic acid. 

A polysaccharide can be conveniently degraded for purposes of structural determinations in a rather simple way. The polysaccharide to be examined is dissolved in an excess of an oxygen-free solution of a base, usually saturated lime-water, and allowed to stand at 25-37° for several months. Cations are then removed from the solution with a suitable cation-exchange resin. Residual polysaccharide may be precipitated with three volumes of ethanol, and the degradation products separated by cellulose-column chromatography, or by fractional reprecipitation of their calcium salts. ... 

Cellulose Column Chromatography, Analysis of Mixtures of Sugars by... 

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