Oligosaccharide isolation

The compositions of high-mannose oligosaccharides isolated from urine of animals suffering from genetic or chemically induced mannosidoses have been assigned from their f.a.b.-mass spectra. After chromatography, some samples were sufficiently pure to be examined underivatized. Others required conversion into their peracetylated derivatives. 

In a study completed during the early development of f.a.b.-m.s., both f.d. and f.a.b. were used to characterize 101 fractions containing neutral oligosaccharides isolated from human milk. Samples were examined as their peracetylated alditols. In subsequent work, the structures of two minor acidic oligosaccharides from human milk were investigated. The per-methylated derivatives were analyzed by f.a.b.-m.s., and their compositions and sequences were defined by the f.a.b. data. Methylation analysis and partial formolysis were the other principal methods used. 

D. Carlson, Structures and immunological properties of oligosaccharides isolated from pig submaxillary mucins, J. Biol. Chem., 243 (1968) 616-626. 

Scheme 40 Large Oligosaccharides Isolated from Asialofetuin (Complex-Type) and Ribonuclease B (High-Mannose-Type) by Hydrazinolysis[1491...

Jia, Z., Cash, M., Darvill, A. G., York, W. S. (2005). NMR characterization of endogenously O-acetylated oligosaccharides isolated from tomato Lycopersicon esculentum) xyloglucan. Carbohydr. Res., 340, 1818-1825. 

Oligosaccharides Isolated from Partial Hydrolyzates of Certain Blood-group Substances... 

Oligosaccharide isolated Source Hydrolysis conditions ences... 

Lipid-linked Oligosaccharides Isolated from Plants... 

Fucose Containing Oligosaccharides Isolated from Human Blood-Group A and B Substances, Nature (1965) 206, 594. 

XLVIII. Activity of Fucose-Containing Oligosaccharides Isolated from Human Urine and from Human Ovarian Cyst A and B Blood Group Substances, /. Immunol. (1971) 106,1572 ... 

Blood Groups XXIV. Some Oligosaccharides Isolated from Dialysates after Mild Acid Hydrolysis of Human Blood Group B Substances from Ovarian Cyst Fluid, J. Amer. Chem. Soc. (I960) 82, 1122. 

Fig. 26.—Structure of Oligosaccharides Isolated from Mannosidosis Urine. (Oligosaccharides 1-3, see Refs. 168-170 oligosaccharides 4-7, see Ref. 170.)...

Fig. 27.—Structure of Oligosaccharides Isolated from Sialidosis Urine (continued on pp. 192 and 93).,7,-,7e...

Fig. 29.—Structure1" of Oligosaccharides Isolated from Urine and Tissues of GMi-gangliosidosis Type I.

Fig. 30.—Structure of Oligosaccharides Isolated from Urine of SandhofFs Dis-...

Fig. 46.—Novel Structures of Oligosaccharides Isolated From Urine of CM, Ganglio-sidosis. ...

Kaczynski, Z., Braun, S., Lindner, B., Niehaus, K., Holst, O. Investigation ofthe chemical structure and biological activity of oligosaccharides isolated from rough-type Xanthomonas campestris pv. campestris B100 lipopolysaccharides. J Endotoxin Res 13 (2007) 101-108. 

Partial-acetolysis studies conducted on yeast D-mannopyranans containing (l- 6)-linked a-D-mannopyranosyl main-chains revealed a large variety of side-chain structures, as represented by oligosaccharides isolated from the acetolyzate (see Table III). Following purification by cellulose-column chromatography, the oligosaccharides were identified by their typical, 13C-n.m.r. spectra.85... 

Compound 22 is a mono-antennary, lower-branch, sialo oligosaccharide, isolated in admixture with 21 from the urine of a patient with sialidosis. The 500-MHz, H-n.m.r. spectrum of the mixture is given in Fig. 20 the set of lower-intensity signals belongs to 22. The n.m.r. parameters for 22 are summarized in Table IX. For the interpretation of the n.m.r. spectrum, comparison with the spectra of 18 and 21 is appropriate. 

Marry M., Cavalier, D.M., Schnurr, J.K., Netland, J., Yang, Z., Pezeshk, V., York, W.S., Pauly, M. and White, A.R. (2003) Structural characterization of chemically and enzymatically derived standard oligosaccharides isolated from partially purified tamarind xyloglucan. Carbohydrate Polymers 51(3), 347-356. 

Fig. 9. Proposed composite structure showing the relationship of the A, B, H, Le , and Le antigenic determinants of the water-soluble human blood group substances from ovarian cysts. The structure of the side chain in brackets has not been established, but the existence of such a chain is indicated by the mechanism of alkaline borohydride degradation and the oligosaccharides isolated. The carbohydrate chain shown is one of many linked to the serines and threonines of the polypeptide backbone. Chains are not homogeneous and may be of various lengths depending upon genetic composition, incomplete biosynthesis, and unknown factors. From Pereira and Kabat, modified from Lloyd and Kabat cf. Kabat. ...

The determinant reacting with anti-I Ma (group 1) is one of the most extensively characterized, using not only oligosaccharides isolated by degradation of the precursor OG substance (Fig. 10), which reacted with all anti-I and anti-i sera, but also with oligosaccharides of various possible alternative structures prepared by chemical synthesis in two laboratories. -... 

Structures of Several Galactomanno-oligosaccharides Isolated from Enzymic Hydrolyzates of Galactomannans... 

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