Cell surface activation markers

Khalfoun B. Thibault G, Lacord M, Gruel Y, Bardos P, Lebranchu Y. Docosahexaenoic and eicosapentaenoic acids inhibit lymphoproliferative responses in viho but not the expression of T cell surface activation markers. Scand J Immunol 1996 43 248-256. 

Toward Nonproliferation-Based In Vitro Assays Cell Surface Activation Markers, Cytokines, Chemokines, and Skin-Homing Receptors... 

In vitro tests for delayed drug reactions include the lymphocyte transformation test and local lymph node assay but nonproliferation-based in vitro assays of cell surface activation markers, cytokines, chemokines, and skin-homing receptors will be increasingly applied. 

The differentiation effect of lycopene was associated with elevated expression of several differentiation-related proteins, such as cell surface antigen (CD14), oxygen burst oxidase and chemotactic peptide receptors (Amir et al., 1999). Recently, it has also been reported that lycopene was also able to stimulate the differentiation marker alkaline phosphatase activity in... 

In resting neutrophils, about 50% of the total cellular FcyRIII pool is expressed on the cell surface. There is considerable variation in this value because many methods used to isolate neutrophils can also inadvertently mobilise these subcellular receptors. The remainder of the total cellular FcyRIII that is not expressed on the plasma membrane is present in the subcellular pool. However, if the FcyRIII normally present on the plasma membrane is cleaved (e.g. via the action of elastase or pronase) and the cells subsequently activated, then FcyRIII reappears on the cell surface via the mobilisation of these pools. Thus, the expression can be restored to up to 70% of the resting level within 15 min via such a translocation. During activation (and presumably priming), FcyRIII (together with other plasma membrane markers) is also translocated to the plasma membrane however, because the receptor is also shed from the cell, the total number of receptors on the cell surface remains largely unchanged. There is also some evidence that continued expression of FcyRIII on the cell surface requires de novo biosynthesis of this receptor (see Fig. 7.8). 

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