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Cell smears, immunostaining

MICROWAVE HEAT-ASSISTED IMMUNOSTAINING OF CELL SMEARS... [Pg.182]

Thin, uniformly spread cell smears are prepared on glass slides and then rehydrated with normal saline for 3 min. This is followed by air-drying for 24 hr and fixation with 0.1% formal saline (1,000ml of normal saline and 2.5ml of 40% formalin) for 2-14 hr postfixation is accomplished with 95-100% ethanol for 10 min. The sections are heated in 10 mM citrate buffer (pH 6.0) in a microwave oven for 5 min at boiling and then allowed to remain in the hot solution for another 5 min before being removed for immunostaining. [Pg.182]

Liu J, Farhood A. Immunostaining for thyroid transcription factor-1 on fine-needle aspiration specimens of lung tumors a comparison of direct smears and cell block preparations. Cancer 2004 102 109-114. [Pg.42]

Even though cell block is the first choice for ICC, there is not always sufficient cell sample for cell block preparation. Therefore, it is necessary to develop techniques that allow multiple immunostaining on limited smears. [Pg.226]

See other pages where Cell smears, immunostaining is mentioned: [Pg.27]    [Pg.29]    [Pg.178]    [Pg.27]    [Pg.29]    [Pg.27]    [Pg.220]    [Pg.222]    [Pg.224]    [Pg.226]    [Pg.228]    [Pg.229]    [Pg.182]    [Pg.55]    [Pg.55]    [Pg.27]    [Pg.220]    [Pg.222]    [Pg.224]    [Pg.226]    [Pg.228]    [Pg.229]   
See also in sourсe #XX -- [ Pg.182 ]



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