Cell recognition events

However, on a higher level of molecular organization, the affinity and specificity of carbohydrate-mediated cell-cell recognition events depend not only on the inherent specificity of one binding site for a specific saccharide epitope but also on factors that are not operative in monovalent interactions. These multivalent factors include, for example, the location of a protein. 

Importantly, this expression pattern was corroborated by flow cytometry measurements (Figure 12.17). This is interesting given that several surface-bound glycan molecules play an important role in cell recognition events. 

The analysis of the packing indicates a well defined hierarchy of intermolecular contacts, some of them suggesting how Le Le interactions may occur in biological conditions providing the molecular basis for cell-cell recognition event. 

Section 25 16 Carbohydrates and proteins that are connected by a chemical bond are called glycoproteins and often occur on the surfaces of cells They play an impor tant role m the recognition events connected with the immune response... 

This diverse set of biosensing experimental demonstrations illustrates the flexibility of the OFRR device. Nearly any biomolecular recognition event can be detected. The examples illustrated with the previously described experiments include DNA sequence detection and virus detection through surface proteins. Additional biosensing examples for which the OFRR is well-suited include site-specific cleavage, protein-protein interactions, and cell genotype/phenotype identification through receptors. Furthermore, as shown by the theory outlined above, the OFRR can be accurately and precisely quantitative. 

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