Site-specific DNA cleavage

PDO 60 site-specific DNA cleavage in the first approach DNA-oligonucleotides covalently bonded to PDO were used [154,155]... 

Austin CA, Patel S, Ono K et al (1992) Site-specific DNA cleavage by mammalian DNA topoisomerase II induced by novel flavone and catechin derivatives. Biochem J 282 883-889... 

Chen, J., Zhang, J., Yang, H. et al. (2010) A strategy for development of electrochemical DNA biosensor based on site-specific DNA cleavage of restriction endonuclease. Biosens. Bioelectron., 26 (1), 144-148. 

Site-specific DNA methylation can also be incorporated into other strategies in which recognition sites are reversibly modulated to offer highly selective cleavage capabilities to the restriction enzymes (see below). 

This approach was the first application of non-enediyne carbon centered radical mediated DNA cleavage agents that were not only capable of binding to DNA but could also be sequence specific. Further work is still needed to elucidate and confirm the sites of cleavage, nature of binding of these molecules and the mechanism of hydrogen abstraction from the nucleic acid backbone. 

This diverse set of biosensing experimental demonstrations illustrates the flexibility of the OFRR device. Nearly any biomolecular recognition event can be detected. The examples illustrated with the previously described experiments include DNA sequence detection and virus detection through surface proteins. Additional biosensing examples for which the OFRR is well-suited include site-specific cleavage, protein-protein interactions, and cell genotype/phenotype identification through receptors. Furthermore, as shown by the theory outlined above, the OFRR can be accurately and precisely quantitative. 

Rep-mediated site-specific cleavage of DNA, ATPase activity and transcriptional repression. EMBO J. 16, 5943-5954. 

Enzymes catalyzing cleavage of DNA, including endo-deoxyribonucleases that generate 5 -phosphomono-esters [EC 3.1.21.x], endodeoxyribonucleases that produce products other than 5 -phosphomonoesters [EC 3.1.22.x], site-specific endodeoxyribonucleases acting on altered bases [EC 3.1.25.x], and exodeoxyribonucleases producing 5 -phosphomonoesters [EC 3.1.11.x]. A few examples are ... 

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