Cell model experimental verification

Karcher, H., Lammerding, J., Huang, H. et al. A three-dimensional viscoelastic model for cell deformation with experimental verification, Biophys. /., 85,3336,2003. 

The safety of the system is analytically determined from the joint contributions of all components. Modeling of thermal and mechanical properties of batteries remains a useful method to approximate their systemic behavior. However, the experimental verification and the (expensive) destructive testing still provide the most valuable data on the actual event progress and propagation. Improvements in the accuracy of thermal models as well as the ever-increasing wealth of data from the past and ongoing testing are expected to improve the reliability of the cell performance predictions. 

Sauer U, Eikmanns BJ (2005) The PEP-pyruvate-oxaloacetate node as the switch point forctubon flux distribution in bacteria. EEMS Microbiol Rev 29 765—794 Schrumpf B, Schwarzer A, Kalinowski J, Puhler A, Eggeling L, Sahm H (1991) A functionally split pathway for lysine synthesis in Corynebacterium glutamicium. J Bacteriol 173 4510-4516 Shiio I, Miyajima R (1969) Concerted inhibition and its reversal by end products of aspartate kinase in Brevibacterium flavum. J Biochem 64 849-859 Shinfuku Y, Sorpitipom N, Sono M, Furusawa C, Hirasawa T, Shimizu H (2009) Development and experimental verification of a genome-scale metaboUc model for Corynebacterium glutamicum. Microb Cell Fact 8 43-57... 

M., Furusawa, C. et al. (2009) Development and experimental verification of a genome-scale metabolic model for Corynebacterium glutamicum. Microb. Cell Fact, 8, 43. 

CaCo-2 cells and HepG2 cells are frequently used as experimental models of the intestine and liver, respectively, results obtained from these cultured cells are not recapitulated in intact animals. These observations highlight the need for verification of the in vivo relevance of in vitro studies by using intact animals. 

However, data from in vitro studies could be misleading and will require verification from animal experiments. In vivo systems are extremely complicated and the interactions of QDs with biological components, such as proteins and cells, could lead to unique biodistribution, clearance, immune response, and metabolism. More importantly, it could lead to predictive models for assessing toxicity. The in vivo research involves experiments performed in the context of the entire system consisting of the body of an experimental animal. The overall behaviour of QDs could be summed up as follow (1) QDs can enter the body via six principal routes—subcutaneous, intravenous, dermal, intraperitoneal, inhalation, and oral (2) QDs can interact with biological components (3) subsequently QDs can distribute to various organs (4) QDs can enter the cells of the organ and reside in the cells. ... 

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