Cell damage shear-sensitive cells

Stirred- tank 1. Flexible and adaptable 2. Wide range of mixing intensity 3. Can handle high viscosity media 1. High power consumption 2. Damage shear sensitive cells 3. High equipment costs... 

Wide range of mixing intensity Damage shear sensitive cells... 

Van der Pol et al. [54] investigated the influence of a silicone AFA on the shear sensitivity of hybridoma cells in sparged cultures. They supplemented the medium with 1 gl poly(ethylene glycol) PEG 6000 and PEG 20,000 to protect the cells from hydrodynamic damage. To suppress the foaming caused by the... 

By contrast to the situation for yeast and bacteria, much of the relevant literature indicates that mammalian, insect, and plant cells are particularly sensitive to damage by hydrodynamic shear forces (32-34). Animal and plant cells are considerably larger than yeast and bacterial cells. They can easily be larger than the size of an eddy and may thus be subjected to the influence of velocity gradients that are sufficiently large to cause damage to these cells. 

Shear Sensitivity of Microcarrier-Anchored Cells In the case of microcarrier-based cell systems, damage to anchored cells ( shear damage ) needs to be explained as due to two distinct effects. These two types are (i) shear damage due to scouring of the microcarrier surface by turbulent eddies and (ii) collision damage ... 

In contrast to many aerobic microbial fermentations, cell cultures do not have critically high oxygen transfer demands. However, mammalian cells are characterized by high shear sensitivity. Consequently, at a large scale, supplementation of air with pure oxygen is required to improve gas transfer without increasing the stirrer speed and power input, which would result in excessive shear and cell damage. 

---