Cell culture models static cultures

Whole animal models and traditional cell culture models have been the primary mode of validation for this purpose. However, animal models are subject to significant expense, lengthy experiments, ethical issues, and it is often difficult to extrapolate results from animals directly to effects on human beings. Results from traditional in vitro cell culture are of limited usefulness due to the difficulty sustaining in vivo functions of primary cells as an isolated, static system [95],... 

In the Licari and Bailey Model [102] and also in the latest Hu and Bentley Model [105] it is proposed that the infection process be described by the Poisson distribution with mean and variance equal to a.MOI. The a-value has been proposed to be dependent on the physical system and a value of a = 0.04 was proposed for static systems [102]. For agitated systems suspension cultures Hu and Bentley proposed a value of a = 0.08 because they state that agitation systems enable higher efficiency of contact between viruses and cells [105]. This is not absolutely true, at least the true reason is not the higher mixing level but the fact that in static cultures, less cell surface is exposed to the virus, since to the cells are attached to a surface. This gives an overall constant of attachment 3-4 fold lower than in suspension systems [61]. 

Heart valve scaffolds with conduit and leaflets made entirely from P3HO-3HH porous films [287] were tested in vitro imder static and pulsatile flow cell culture conditions [44,281]. A larger number of vascular cells, together with the formation of an oriented and confluent cell layer, was found on scaffolds exposed to pulsatile flow [281]. The P3HO-3HH heart valves, preseeded with vascular cells under static conditions, were tested for up to 17 weeks in the pulmonary position in a lamb model. The valves showed good functionality... 

Zhao F, Pathi P, Grayson W, Xing Q, Locke BR, Ma T. 2005. Effects of oxygen transport on 3-d human mesenchymal stem cell metabolic activity in perfusion and static cultures Experiments and mathematical model. Biotechnol Rro 21(4) 1269-80. 

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