Cell culture analog

The Cell Culture Analog Macro and Micro Designs 707... 

Our laboratory has fabricated several generations of micro cell culture analog (pCCA) devices, also called animal-on-a-chip or body-on-a-chip . In... 

Figure 25.2 Illustration of multiple-type cell co-culture systems (a) Random mixed, (b) contact patterning with fuzzy boundaries, (c) co-culture without cell contact on a flat surface, (d) in chambers, (e) the trans-well system, and (/) the micro cell culture analog ( xCCA) device.

The cell culture analog (CCA) system is one approach attempting to narrow this gap. It is developed to address the issues of reactive metabolite formation and tissue-tissue interaction through exchange of metabolites or signal molecules, and to evaluate potential efficacy and toxicity of pharmaceuticals and environmental chemicals. A CCA device can be considered as a simplified and minimized human/animal body, in which mammalian cell cultures are used to represent key functions of specific organs, and cell culture medium is used as a blood surrogate. 

Viravaidya K, Sin A, Shuler ML. Development of a microscale cell culture analog to probe naphthalene toxicity. Biotechnol Prog 2004 20 316-23. 

Viravaidya K, Shuler ML. Incorporation of 3T3-L1 cells to mimic bioaccumulation in a microscale cell culture analog device for toxicity studies. Biotechnol Prog 2004 20 590-7. 

Sin A, Chin KC, Jamil MF, Rostov Y, Rao G, Shuler ML. The design and fabrication of three-chamber microscale cell culture analog devices with integrated dissolved oxygen sensors. Biotechnol Prog 2004 20 338-45. 

Shuler ML, Ghanem A, Quick D, Wong MC, Miller P. A self-regulating cell culture analog device to mimic animal and human toxicological responses. Biotech-nol Bioeng 1996 52 45-60. 

Mahler, G.J., Esch, M.B., Glahn, R.R, and Shuler, M.L. 2009. Characterization of a gastrointestinal tract microscale cell culture analog used to predict drug toxicity. Biotechnol Bioeng, 104(1) 193-205. 

With the uptake of biomass, energy is stored whereby this seems to happen periodically. All in all, this energy should depend on the mean cell density. The density should thus characterise the mean-field properties of a cell culture analogously to a complex viscoelastic modulus . 

Uncoupling Resistances Use in Physiologically Based Pharmacokinetics Models and Cell Culture Analog Systems... 

Sweeney, L.M., Shuler, M.L., Babish, J.G., and Ghanem, A. (1995). A cell culture analog of rodent physiology application to naphthalene toxicology. Toxicol. In Vitro 9 307. 

Background Cell Culture Analog Concept Prototype CCAs Models of Barrier Tissues and Their Use with pCCAs Future Prospects Defining Terms References... 

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