Cell-compatible surfaces

Cell Culture. To achieve a more cell-compatible surface environment, the entire particle grachent was dipped into a PLL-g-PEG-RGD solution. The RGD-peptide sequence is end-grafted to the PLL-g-PEG copolymer and is known to promote cell attachment by interacting with cell integrins. Rat calvarial osteoblasts (RCO) were cultivated for 7 days on a pMticle gradient with the following seeding conditions 6000 cells/cm in a-DMEM with 10% fetal bovine serum Mid 1% antibiotics medium, incubation at 37 °C, 7% CO2,100% humidity. After 7 days of culture, the cells were fixed,... 

The conclusion drawn from the above-discussed features of native endothelium is that—where feasible—construction of host endothelium on the surface of synthetic implants—i.e. engineered endothelialization—may present a promising pathway for fundamentally resolving the biocompatibility and bio-functionality of artificial cardiovascular transplantation. This proposed biocompatibility is, however, not simply thromboresistance (or blood compatibility) plus blood-cell compatibility, but a real bioactive, self-renewable and specifically functional alliance of tissues and synthetics. 

Immobilization of chromatophores on the surface of the microbeads represents a key step in biosensor development. Fish chromatophores are anchorage-dependent cells that require compatible surface for attachment, subsequent spreading, and growth [5]. Generally, immobilization on the surface of small beads or microcarriers is a suitable method for the cultivation of anchorage-dependent cells. Van Wezel [13] first reported immobilization of cells on mammalian cells on diethyla-minoethyl (DEAE)-Sephadex A50. Subsequently this type of carrier was improved by optimizing... 

Another study aiming to impart cell adhesion properties to PVA-C made use of a recently synthesized novel poly(amic acid) (PAA) polymer that has been shown to be cell compatible to form a PAA-grafted/crossIinked-PVA hydrogel (PAA-g/c-PVA). Functionalization of the PVA-C surface was accomplished by grafting of the PAA onto it to provide sites for cell attachment (Fig. 15). Successful endothelization with vascular endothelial cells was demonstrated (Fig. 16) [111]. 

Lee JN, Jiang X, Ryan D, Whitesides GM. Compatibility of mammalian cells on surfaces of poly(dimethylsiloxane). Langmuir 2004 20 11684-91. 

Evans et al. (1991) examined the interaction of DLC obtained using a saddle field source and baby hamster kidney cells and reported good cell adhesion on the coated surfaces, indicating good cell compatibility. 

In the try it and see approach a great deal of experimentation is still required in order to obtain the optimal chemical and structural design of compatible surfaces. However, by modeling the cell migration in the context of the materials and peptides of interest, more comprehensive predictive models can be developed and used to design functional materials for specific applications. 

Also, PLLA/grayi-HAp materials had better mechanical properties than corresponding PLLA/HAp composites. At a gro -HAp (g-HAp) content of 4 wt%, the PLLA/g-HAp nanocomposite showed a maximum in tensile strength, bending strength and impact energy. The PLLA/g-HAp composites also demonstrated improved cell compatibility due to the good biocompatibility of the HAp nanoparticles and the more uniform distribution of the g-HAp nanoparticles on the film s surface [147]. 

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