Cell-Based Technologies

In contrast to enzyme assays, cell-based assays present the target in a more physiological milieu. With enzyme assays, it may be difficult to purify and express active kinases and phosphatases in their full-length forms and they may require the use of fusion proteins with kinase activity domains. Cell-based technologies, on the other hand, present the opportunity to express the targets with regulatory domains included. Furthermore, cell-based assays usually detect only cell-permeable inhibitors and have the potential to identify more unusual mechanisms, as described earlier. 

FIGURE 1.2 (See color insert following page 114.) Cell-based assay concepts for kinase and phosphatase HTS. 

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Every few decades a medical innovation is perfected that profoundly influences the practice of medicine. Widespreadvaccinationagainstcommoninfectious agents and the discovery of antibiotics serve as two such examples. Many scientists now believe that the potential of nucleic-acid- and cell-based technologies rivals even the most significant medical advances achieved to date. 

FuelCell Energy is a partner with Versa Power Systems, Nexant, and Gas Technology Institute to develop more affordable fuel-cell-based technology that uses synthesis gas from a coal gasifier. The key objectives include the development of fuel cell technologies, fabrication processes, and manufacturing capabilities for solid oxide fuel cell stacks for multi-mega-watt power plants. 

Tam KY. Potential of Using Cell Based Technology for Predicting Bioavailability. Amsterdam Dissolution, Bioavailability Bioequivalence, 2003. 

GENERAL DEVELOPMENT OF A FUEL CELL-BASED TECHNOLOGY... 

Mrksich, M. Cell-based technologies nonmedical applications. In WTEC Panel on Tissue Engineering Research-. Final Report, Mclntire, L.V., Ed. Academic Press New York, 2002 61-69. 

Caplan, A.L et al. Cell-based technologies for cartilage repair, in Biology and Biomechanics of the Traumatized Synovial Joint The knee as a Mode/, Finerman,G.A.M. and Noyes, F.R. (Eds.), American Academy of Orthopaedic Surgeons, Rosemont, pp. 111-122,1992. 

The resulting architectures can finally be mapped either into modular bit-sliced macro cells or in a standard-cell based technology [Not90]. In this process, logic synthesis is applied to generate the controller modules. These phases will not be described in this paper. 

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