Cell adhesion guided

Fig. 4 The effect of proteins on cell adhesion, (a) Kretschmann configuration for SPR. (b) Reflectance (R) as a function of incident angle (9), before (black) and after (red) the adsorption of substances, (c) Left. Time course of SPR angle shift during exposure to culture medium supplemented with 2% FBS (solid line) and the fraction of adherent cells determined by TIRFM (circles) on NH2-SAM. The dashed line is a manual fit to the symbols, included simply as a guide [42]. Right The concentrations of serum proteins in FBS...

Protein-Decorated Surfaces for Spatially Guided Cell Adhesion. 47... 

Note that the above-mentioned, rather simplified set of characteristics for a biomimetic strategy of guided cell adhesion were first developed for planar surfaces. For many cell-types, this accounts for an interesting model system with a sufficient relevance to the biological situation however, there also exist a number of cell types that might behave differently with respect to surface attachment, growth,... 

Compared to systems that rely on protein adsorption for spatially guided cell adhesion, reports on cell chips based on peptide-mediated adhesion are less frequent, but emerging. In principle, identical methods to those used for the microstructuring of proteins can be employed for peptides, although reports on direct patterning (controlled deposition of peptides) prevail. 

Cell studies on scaffolds of nano- and submicrometer-scaled fibers have shown that these dimensions promote not only cell adhesion, but also have beneficial effects on proliferation and differentiation of cells [174-177], These effects are more prominent with decreasing fiber diameters. It seems relevant that the cells can be guided and bridged by the artificial fibers. Meshes with aligned fibers are particularly promising, e.g., for guiding the growth of nerve cells (Fig. 8) [178],... 

Topography of a substrate is as important as chemical composition in controlling cell responses and functions [69]. Structural properties of the substrate at molecular, nanometer, and micrometer scales control and influence cell adhesion, spreading, migration, growth, differentiation, and a variety of functions, in a cell-type specific manner [24], With nano and microfabrication techniques, we are now able to mimic in vivo structures, create new topographic characteristics at the cellular and subcellular level, and guide cells to behave in the way we prefer. 

Polymers tiiat can guide development of tissue through the addition of arginine-glycine-aspartic acid (RGD) or otirer polypeptide sequences have also been fabricated. The RGD sequence of amino acids is foimd in proteins of the extracellular matrix such as fibronectin, and one of the sequence s roles is to promote cell adhesion. The purpose of synthesizing such polymers is to manipulate the body into treating the syntiretic material, which mimics the extracellular matrix, like natural tissue. If successful, cells should then readily attach and proliferate on the scaffold. Shakesheff and co-workers provide a review of the three major techniques used to create polymer-peptide hybrid materials. ... 

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