Cell adhesion collagen

Keywords Cell adhesion Collagen Fibronectin - Focal adhesions Integrins... 

NAP-I, NAP-2 Neutrophilactivating peptides -1 and -2 NBT Nitro-blue tetrazolium NCI Non-collagen 1 N-CAM Neural cell adhesion molecule... 

In order to achieve the firm fixation of the artificial cornea to host tissues, composites of collagen-immobilized poly(vinyl alcohol) hydrogel with hydroxyapatite were synthesized by a hydroxyapatite particles kneading method. The preparation method, characterization, and the results of corneal cell adhesion and proliferation on the composite material were studied. PVA-COL-HAp composites were successfully synthesized. A micro-porous structure of the PVA-COL-HAp could be introduced by hydrochloric acid treatment and the porosity could be controlled by the pH of the hydrochloric acid solution, the treatment time, and the crystallinity of the HAp particles. Chick embryonic keratocyto-like cells were well attached and proliferated on the PVA-COL-HAp composites. This material showed potential for keratoprosthesis application. Further study such as a long-term animal study is now required [241]. 

COLl domain of type XXIII collagen contains multiple copies of a conserved KGD motif used for integrin-mediated cell adhesion by collagen type XVII. ... 

The data concerning the effect of fluoride-containing apatites on cell adhesion, proliferation and expression seem rather disparate, probably because of the variation of other surface characteristics and the use of different cell strains. No real negative effect of fluoride-containing substrates has been reported so far and the osteoblast cells seem to behave similarly [184,185] or better [183,186,187] on fluoridated apatites than on HA surfaces. Some reports mention weaker attachment and proliferation, compensated for by improved collagen matrix production [188]. The shape of cells appeared different on fluoridated apatites than on HA. Other authors have found an improvement in cell attachment [183] which has been attributed to the change in surface charge of FA compared to HA. 

Sacca, B., Fiori, S., and Moroder, L. (2003). Studies of the local conformational properties of the cell-adhesion domain of collagen type IV in synthetic heterotri-meric peptides. Biochemistry 42, 3429-3436. 

Type IV collagen is a fundamental component of basement membranes (Fig. 1A). Basement membranes represent the portion of extracellular matrix that remains in direct contact with its formative cells. Basement membranes play an important role in cell adhesion, growth and differentiation, tissue repair, molecular ultrafiltration, cancer cell invasion, and metastasis. 

R10. Ridley, R. C., Xiao, H., Hata, H., Woodliff, J., Epstein, J., and Sanderson, R. D., Expression of syndecan regulates human myeloma plasma cell adhesion to type I collagen. Blood 81,161—11A (1993). 

The use of plastic materials for routine cell culture on the laboratory scale was introduced at the end of the 1960s, and some characteristics of glass surface, such as hydrophobicity and negative charge, were maintained in these materials. Polystyrene is the most widely used plastic material for animal cell adhesion at present, because of its surface characteristics, its low cost, and also its transparency. For more demanding cell lines, the surface has to be submitted to a treatment that involves coating with proteins such as poly-lysine, poly-ornithine, or extracellular matrix-derived proteins such as fibronectin, laminin, and collagens. 

Microcarriers are small particles, made of materials such as cellulose, dextran, glass, collagen, or gelatin. Generally, they have a spherical shape and present a surface structure and composition that promotes cell adhesion and growth. 

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