Catalase iron content

Catalase contains protoporphyrin IX and in addition variable quantities of biliverdin, depending on the method of preparation. Catalase containing biliverdin is still catalytically active. The molecular weight is about 250,000, and the iron content is about 0.09%. Each molecule therefore... 

The iron content of the body is 4—5g. Most of it is present in the hemoglobin (blood) and myoglobin (muscle tissue) pigments. The metal is also present in a number of enzymes (peroxidase, catalase, hydroxylases and flavine enzymes), hence it is an essential ingredient of the daily diet. The iron requirement depends on the age and sex of the individual, it is about 1.5-2.2 mg/day. Iron supplied in the diet must be in the range of 15 mg/day in order to meet this daily requirement. The large variation in intake can be explained by different extents of absorption of the various forms of iron present in food (organic iron compounds vs simple... 

Overproduction of free radicals by erythrocytes and leukocytes and iron overload result in a sharp increase in free radical damage in T1 patients. Thus, Livrea et al. [385] found a twofold increase in the levels of conjugated dienes, MDA, and protein carbonyls with respect to control in serum from 42 (3-thalassemic patients. Simultaneously, there was a decrease in the content of antioxidant vitamins C (44%) and E (42%). It was suggested that the iron-induced liver damage in thalassemia may play a major role in the depletion of antioxidant vitamins. Plasma thiobarbituric acid-reactive substances (TBARS) and conjugated dienes were elevated in (3-thalassemic children compared to controls together with compensatory increase in SOD activity [386]. The development of lipid peroxidation in thalassemic erythrocytes probably depends on a decrease in reduced glutathione level and decreased catalase activity [387]. 

The brain is prone to suffer oxidant damage due to its relatively low content of antioxidant enzymes (Carrillo et al. 1992) and its high content of iron, as shown in neuromelanin by Laser microprobe mass analysis (LAMMA) (Good et al. 1992). Immu-noreactive catalase protein decreased in the hypothalamus and the prefrontal cortex of the ageing rat brain (3 vs. 12 months) by 63 and 55 %, respectively (CiRiOLO et al. 1997). Iron will be easily released when the cells are injured and cannot be safely bound as cerebrospinal fluid has not any significant iron binding capacity. The toxicity of 02 " has been... 

Catalase. Crystallization of beef liver catalase and its molecular weight. Sunilaiity of various animal catalases. Total iron and heme iron in catalase. Amino acid content of horse liver catalase. 

The amino acid content of horse liver catalase is reported to be as follows (per gram atom of iron) total number of amino acid residues, 496 histidine, 15 or 16 arginine, 27 lysine, 32 (220). 

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