Catabolite gene activating protein

However, the a helices are not packed against each other in the usual way as described in Chapter 3. Instead, a helices 2 and 3, residues 15-36, form a unique helix-turn-helix arrangement that in 1981 had only been observed once, in a different bacterial DNA-binding protein, the catabolite gene-activating protein CAR... 

CAP catabolite gene activator protein GSSG glutathionine disulfide... 

CAP. The catabolite gene activator protein, sometimes incorrectly referred to as the CRP protein. The latter term, in small letters (crp), should be used to refer to the gene but not to the protein. 

McKay, D. B., and Steitz, T. A. (1981). Structure of catabolite gene activator protein at 2.9 A resolution suggests binding to left-handed B-DNA. Nature 290, 744—749. 

Know the roles of a regulator gene, repressor and corepressor, catabolite gene activator protein (CAP), cyclic AMP, and attenuator. 

Recall that the lacl, crp, and cya genes encode the Lacl repressor protein, the catabolite gene activator protein, and adenylate cyclase, respectively. 

Schultz, S.C., Shields, G.C., Steitz, T.A. Crystallization of Escherichia coli catabolite gene activator protein with its DNA binding site. The use of modular DNA. J. Mol. Biol. 1990, 213(1), 159. 

Fig. 15.11. Ribbon diagrams [54]. Left calcium-binding motif (residues 78-108) of carp parval-bumin (4CPV) right DNA binding motif (residues 168-191) of catabolite gene activator protein (3 GAP)...

Notes 1. Abbreviations used CAP, catabolite gene activator protein (also called cAMP receptor protein, CRP) RNAP, RNA polymerase. 2. Operon Database (DDE) at http //odb.kuict.Kyato-u.ac.jp/ is the data retrieval system for known operons of completed genomes. 

Bacteria Escherichia coli Release of glucose inhibition of enzyme induction (see Catabolite repression). Initiation of mRNA synthesis mediated by a specific cAMP receptor protein (catabolite gene activator protein). Inhibition of the degradation of mRNA bound to ribosomes. Stimulation of the synthesis of many enzymes... 

G. Zubay, D. Schwartz, and J. Beckwith [27]. Catabolite gene activator protein (CAP) partially purified and characterized. 

The cell-free system allows the fractionation of components found in normal bacterial strains and comparison of these strains to mutated ones. Thus a new protein, termed the catabolite gene activator protein (CAP), has been partially purified and found to be present in normal strains. The interaction of this protein with the lac system and its role in control with be discussed in Section IV. 

This factor, which has been named catabolite gene activator protein (CAP), is a basic protein with a molecular weight of 45,000. When subjected to SDS-polyacrylamide gel electrophoresis, CAP dissociates into a single polypeptide chain of molecular weight, 22,000. CAP binds cyclic AMP with a formation constant of about 0.6 x 10 liters moles . Attempts to detect CAP binding to core RNA polymerase or to the... 

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