Capillary zone electrophoresis, description

Schmitt-Kopplin, P., Garmash, A. V., Kudryavtsev, A. V., Perminova, I. V., Hertkorn, N., Freitag, D., and Kettrup, A. (1999b). Mobility distribution description of synthetic and natural polyelectrolytes with capillary zone electrophoresis. /. AOAC Int. 82,1594-1603. 

To date, the use of chemometrics for method development and robustness testing has been published for all areas of CE, including capillary zone electrophoresis (CZE), capillary electrokinetic chromatography (EKC) using chiral selectors for enantioseparations, micellar electrokinetic chromatography (MEKC), and microemulsion electrokinetic chromatography (MEEKC). A comprehensive description can be found in Chapters 5 and 13 as well as in recent reviews (6-11). Several monographs on chemometrics in analytical chemistry have been published such as References 12-14. This chapter will... 

The five major formats of the CE experiment are capillary zone electrophoresis (CZE), micellar electrokinetic chromatography (MEKC), capillary gel electrophoresis (CGE), capillary isoelectric focusing (CIEF) and capillary iso-tachophoresis (CITP). The following gives a brief description of the mode of separation, and some examples of analyses which can be performed employing such separation strategies. 

Description of System and its Basic Operating Characteristcs A schematic of the capillary zone electrophoresis (CZE) system is... 

Reijenga, J.C. and Kenndler, E., Computational simulation of migration and dispersion in free capillary zone electrophoresis. I. Description of the theoretical model, J. Chromatogr., 659, 403, 1994. 

Kenndler, E. Dependence of analyte separation on electro-osmotic flow in capillary zone electrophoresis Quantitative description by the reduced mobility. J. Microcol. Separ. 1998, 70(3), 273-279. 

One of the major advantages of CE as a separation technique is the wide variety of separation modes available. Analytes can be separated on the basis of charge, molecular size or shape, pi, or hydrophobicity. The same CE instrument can be used for zone electrophoresis, IEF, sieving separations, isotachophoresis, and chromatographic techniques such as MEKC and capillary electrokinetic chromatography. This section provides a brief description of each separation mode. Zone electrophoresis, IEF, and sieving are the primary modes used for protein separations, and these will be discussed in detail in the following sections. 

The movement of soil colloidal particles was the first description of electrophoresis as early as 1809. However, Arne Tiselius ( 1937) was the first to construct a successful instrument useful for the separation of serum protein by electrophoresis using the boundary separation principle. Because of the clinical significance of this type of separation, many improvements and refinements followed, such as utilizing paper, cellulose acetate, gel, and more recently capillaries in order to speed up and better separate (into distinct zones) the different proteins. The electric current can be utilized in the clinical applications to accomplish not just separation but other tasks ... 

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