Caliper/Agilent

Caliper/Agilent markets DNA chips which have become standard analytical tools for a number of life science laboratories. Commercial DNA chips are also available from companies such as Affymetrix and GE Healthcare. Microfluidic chips for carrying out purification processes for nucleic acids have been reported Cell isolation and lysis as... 

Today, Caliper Life Sciences, MA, USA [255] and Agilent Technologies, CA, USA [256] offer microfluidic chips for DNA and Protein analysis. Liquid propulsion is provided via electroosmosis and combined with capillary electrophoretic separation. The sample is electroosmotically transported and metered inside the chip, then separated via capillary electrophoresis and analysed by fluorescence detection. (Fig. 14). The whole assay is performed within minutes, instead of hours or days. 

Commercially, both Agilent Technologies and Caliper LifeSciences now cell CE microchips. 

Caliper Technologies and Agilent have developed an annexin V assay in a microfluidic system, which allows flow cytometric analysis of apoptosis with a minimal number of cells [6, 7]. In this setup, the cells are moved by pressure-driven flow inside a network of microfluidic channels and are analyzed individually by two-channel fluorescence detection. As only a small number of cells (as few as 50-100) are consumed per assay, this setup is particularly suitable for working with cells of limited availability, for example, primary cells. The system has been applied to evaluate staurosporine-induced apoptosis and annexin V binding in human umbilical vein endothelial cells (HUVECs) and normal human dermal fibroblasts (NHDFs). The results are in good... 

Gas chromatography-mass spectrometer Agilent 7890 GC/5973 MS (Agilent United States), Electric thermostatic drier (Shanghai Yuejin Medical Machinery Factory), Rotary Evaporator Model RotavaporR-200 (BUCHI), Model TurboVap II (Caliper), Ultrasonic cleaner Model KQ-700E (Kunshan Ultrasonic Instrument Co., Ltd.). 

Numerous manufacturers of analytical instruments and other products and services related to analytical chemistry have contributed by providing diagrams, application notes, and photos of their products. We are particularly grateful to Agilent Technologies, Bioana-lytical Systems, Beckman Coulter. Inc.. Brinkman Instruments, Caliper Life Sciences, Hach Co., Hamamatsu Photonics, InPhotonics, Inc, Kaiser Optical Systems, Leeman Labs, LifeScan, Inc., Mettler-Toledo, Inc., National Instruments Corp.. Ocean Optics, Inc., Perkin-Elmer Corp., Postnova Analytics, Spectro Analytical Instruments, T. A. Instruments, Thermo-Electron Corp., and Varian, Inc. lor providing photos. 

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