Buffers Butylamine

Fig. 3.42 NMR-NOE spectra of 3,5-dichloro-benzyl-N-butylamine and 3,5-dichlorobenzyl-N-hexylamine in deuterated phosphate buffer...

Beilstein Handbook Reference) AI3-03947 Aminomethyl propanol Aminomethylprop-anol AMP AMP 75 AMP 95 AMP Regular BRN 0506979 Caswell No. 037 Corrguard 75 EINECS 204-709-8 EPA Pesticide Chemical Code 005801 HSDB 5606 Hydroxy-tert-butylamine lsobutanol-2-amine KV 5088 NSC 441. Boiler water treatment ohemioal, corrosion inhibitor, carbon dioxide absorber. Widely used as a buffer and phosphate acceptor in assay of phosphatases. Suitable as buffer for manual and automated determination of alkaline phosphatase using 4-nitrophenyl phosphate as substrate. Solid mp = 25.5° bp = 165.5° d O = 0.934 soluble in CCI4, freely soluble in H2O LDso (rat orl) = 2900 mg/kg. Lancaster Synthesis Co. Lancaster Synthesis Ltd. 

Mobile phase MeCN buffer 50 50 (Buffer was 10 mM NaH2P04 -1-1.2 mL/L butylamine, pH adjusted to 3 with phosphoric acid.)... 

Mobile phase MeCN THF buffer 7 6 87 (Buffer was 0.8% acetic acid containing 5 mM sodium hexanesulfonate, 10 mM di-n-butylamine, and 0.12% phosphoric acid, pH 3.3.)... 

Figure 11-25 NMR-NOE spectra of 3,5-dichlorobenzyl-7V-butylamine and 3,5-dichlorobenzyl-N-hexylamine in deuterated phosphate buffer in the absence a, c and presence b, d of lecithin (reproduced from [99] with permission from Wiley-VCH, D-69451 Weinheim, Germany).

For routine work commercially available mixtures are useful. These contain several components and the addition of specified amovmts of a strong base is all that is required for the preparation of the buffer solution. Among these is Britton-Robinson buffer (Table 1), which contains acetic, phosphoric, and boric acids. Universal buffers for spectrophotometry may contain such components as chloroacetic, formic, acetic, phosphoric, succinic, citric, boric acids, tris(hydroxymethyl)aminomethane, and butylamine. These buffers are transparent at wavelengths at least down to 240 nm. 

Yang and Thyrion [1072] studied peak shape and retention on a wide range of basic amine-containing compounds procaine, adiphenine, drofenine, nafronyl, tetracaine, meclofenoxate, 4-aminobenzoic acid, and caffeine. The basic mobile phase was 65/35 acetonitrile/water (20 mM acetate buffer at pH 4.5) and the column a Ci8 (A =260 and 280 nm). The effect of the identity (i.e., di-n-butylamine and triethylamine) and concentration of mobile phase modifier from 0 to 0.3% was studied. Retention times from 2 to 20 min resulted and asymmetry factors in general were <2.5. 

Moeton and Rogees [49] have been able to distinguish various relabelled soluble ribonucleic acids through TLC on PEI-cellulose and other ion exchangers. Their solvents were the upper phase of the system isopropanol-formamide-phosphate buffer, pH 6.2 (20 + 5 + 60) [32] or the lower phase of the mixture n-butanol-water-tri-n-butylamine-acetic acid-di-n-butyl ether (100 + 130 + 10 + 2.5 + 29) [107] the fractions were detected by autoradiography [49]. 

Figure 10.8 Effect of pH on the inhibition of tryptic activity by cetyl dibenzylammonium ions (curve A) or butylamine (curve B) and in the absence of the inhibitor (curve C)at 25 C in 0.1 M phosphate buffer. The concentration of the inhibitors was 3.8 mM. The activity obtained in the absence of the inhibitor at pH 7.5 was taken as 100 %. From Nakaya et al, [60] with permission.

Aluminium resists methyl iodide CH3I at room temperature and in the absence of humidity. In the presence of water, methyl iodide releases hydriodic acid HI, which attacks aluminium. The addition of an alkaline buffer such as dimethylamine or butylamine, which neutralises the released acid, will prevent this attack. 

Dealing with substrate inhibition caused by application of higher amounts of reachon educts needs further investigahons in terms of ideal reaction conditions and amino donor/acceptor ratio. One crucial step for successful transamination is the choice of a suitable amino donor. Fesko et al. evaluated the influence of several amino donors and substrates to investigate a one-enzyme system [41], The influence of the amino donor on the ee was shown by examination of reactions catalyzed by the co-transaminase from Paracoccus denitrificans. Usually low ee values were obtained with alanine as the amino donor. The best results were acquired with l-phenoxy-2-propanone and 1-phenylethylamine in sodium phosphate buffer pH 7.5 at 30 °C. To get higher substrate loading, it is necessary to add cosolvents due to the low solubility of the substrates. In this study, cyclohexane and sodium dodecyl sulfate (SDS) were best cosolvents. For the preparation of (R)- and (S)-phenoxypropane-2-amine in a similar yield, three enzymes were used for the synthesis [66] rac-2-butylamine as amino donor usage was increased by tenfold (500 mM). 

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