Paracoccus denitrificans

Iwata, S., Ostermeier, C., Ludwig, B., Michel, H. Structure at 2.8 A resolution of cytochrome c oxidase from Paracoccus denitrificans. Nature 376 660-669, 1995. 

Fig. 12. EPR spectra of the Rieske fragment from the 6ci complex of Paracoccus denitrificans (ISFpd, top) and of the Rieske-type ferredoxin from benzene dioxygenase (FdBED, bottom). EPR conditions were as follows (ISF/FdBEn) microwave frequency, 9.021 GHz modulation amplitude, 1 mT/0.9 mT microwave power, 1 mW/9 mW temperature, 15 K/30 K.

Fig. 15. EPR spectra of the Rieske cluster in membranes of Paracoccus denitrificans in different redox states of the quinone pool and with inhibitors added. Q x, ascorbate reduced Qred) reduced with trimethylhydroquinone dissolved in dimethyl sulfoxide +EtOH, reduced with trimethylhydroquinone dissolved in 90% ethanol +Myxo, ascorbate reduced with myxothiazol added + Stigma, ascorbate reduced with stigmatellin added. Only the gy and signals are shown. The dotted line has been drawn at...

Fig. 17. Cyclic voltammogram of the water-soluble Rieske fragment from the bci complex of Paracoccus denitrificans (ISFpd) at the nitric acid modified glassy carbon electrode. Protein concentration, 1 mg/ml in 50 mM NaCl, 10 mM MOPS, 5 mM EPPS, pH 7.3 T, 25°C scan rate, 10 mV/s. The cathodic (reducing branch, 7 < 0) and anodic (oxidizing branch, 7 > 0) peak potentisds Emd the resulting midpoint potential are indicated. SHE, standEU d hydrogen electrode.

Grajek, W.H. and WaUcowiak-Tomczak, D., Factors influencing the denitrification rate of red beet juice by the bacteria Paracoccus denitrificans, J. Agric. Food Chem., 45, 1963, 1997. 

Koenig K, JR Andreesen (1991) Aerobic and anaerobic degradation of furan-3-carboxylate by Paracoccus denitrificans strain MK33. Arch Microbiol 157 70-75. 

Strains of some facultatively heterotrophic and methylotrophic bacteria can use CS2 as sole energy source, and under aerobic conditions also COS, dimethyl sulfide, dimethyl disulfide, and thioacetate (Jordan et al. 1995). It was proposed that the strains belonged to the genus Thiobacillus, though they are clearly distinct from previously described species, and they have now been assigned to Paracoccus denitrificans (Jordan et al. 1997). 

Jordan SE, IR McDonald, AJ Kraczkiewicz-Dowjat, DP Kelly, FA Rainey, J-C Murrell, AP Wood (1997) Autotrophic growth on carbon disulfide is a property of novel strains of Paracoccus denitrificans. Arch Microbiol 168 225-236. 

Ostermeier C, Harrenga A, Ermler U, Michel H. 1997. Structure at 2.7 A resolution of the Paracoccus denitrificans two-subunit cytochrome c oxidase complexed with an antibody Ev fragment. Proc Natl Acad Sci USA 94 10547. 

Table 5. Flow rates in a two-stage continuous cultivation of Paracoccus denitrificans with acetic acid as substrate ...

Paracoccus denitrificans, a facultatively methylotrophic bacterium, is able to grow and accumulate PHAs on methanol. Recombinant P. denitrificans strains with increased expression levels of all PHA synthetic enzymes were investigated for the enhanced production of PHA. The PHA content and PHA accumulation rate of recombinant P. denitrificans with homologous overexpression of PHA synthase were 2 and 2.7 times higher, respectively, than those of the wild strain, suggesting that the step of PHA synthase was limited in PHA biosynthesis [111]. 

Albracht, S.P.J., Van Verseveld, H.W., Hagen, W.R., and Kalkman, M.L. 1980. A comparison of the respiratory chain in particles from Paracoccus denitrificans and bovine heart mitochondria by EPR spectroscopy. Biochimica et Biophysica Acta 593 173-186. 

Obviously the redox poise in biological systems is very important and the movement of selenium through this process has been investigated for denitrifiers such as Paracoccus denitrificans,159 a specialized selenate-respiring bacterium Thauera selenatis which used selenate as the sole electron acceptor,160,161 and phototrophic bacteria which produced different reduced forms of selenium when amended with either selenite or selenate and even added insoluble elemental Se.162 As noted above, Andreesen has commented on the importance of redox active selenocysteines135 and Jacob et al.136 note the importance of the thioredoxin system to redox poise. 

Rainey FR, Kelly DP, Stackebrandt E, et al. 1999. A re-evaluation of the taxonomy of Paracoccus denitrificans and a proposal for the combination Paracoccus pantotrophus comb. nov. Int J Syst Bacteriol 49 645-51. 

The solution structures of the reduced and oxidized forms of a biologically fully active 10.5 kDa fragment of the Paracoccus denitrificans cytochrome c-552 enriched with and N isotopes were obtained. No significant... 

Bergeron RJ, Dionis JB, Elliot GT, Kline SJ (1985) Mechanism and Stereospecificity of the Parabactin-mediated Iron-Transport System in Paracoccus denitrificans. J Biol Chem 260 7936... 

Peterson T, Neilands JB (1979) Revised Structure of a Catecholamide Spermidine Siderophore from Paracoccus denitrificans. Tetrahedron Lett 4805... 

Paracoccus denitrificans, 45 352 respiratory chain, 45 353-362 Paracoccus versutus, electron transfer chain, 45 351-401 Paraelement defined, 28 169... 

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