Brucella growth

Noncultivable or slow-growing organisms (Histoplasma capsulatum, Bartonella or Brucella species), in which cultures are often discarded before sufficient growth has occurred for detection... 

Drazek, E. S., Houng, H.-S.H., Crawford, R.M., Hadfield, T.L., Hover, D.L., Warren, R. 1995. Deletion of purE attenuates Brucella melitensis 16M for growth in human monocyte-derived macrophages. Infect. Immun. 63 3297-3301. 

Jiang X, Baldwin CL. Effects of cytokines on intracellular growth of Brucella abortus. Infect Immun. 1993 61 (1) 124-134. 

Brucella are very fastidious organisms requiring a rich growth medium. They are also slow growing, and media are therefore supplemented by a cocktail of... 

The double stranded polynucleotide, poly A U, when administered intraperitoneally at the same time as intravenous infection with Brucella abortus, suppressed the growth of that organism in the spleen and Uver of mice (Madraso and Cheers 1978). The antiviral function of spleen cells induced by poly A U was evident in the supernatant fluid when cultured for 48 h at 37 C (Lee et al. 1992). Murine cytomegalovirus-induced plaques were reduced to 52 and 5 % of controls in the plaque assays performed at 37° and 40 , respectively. 

McCullough and Dick (227) have reported similar findings with Brucella suis, and later (228) observed acceleration of growth of certain Br. abortus strains. 

---