Broth dilution susceptibility testing

Dilution susceptibility testing methods are used to determine the minimal concentration of antimicrobial required to inhibit or kill the microorganism. This can be achieved by dilution of an antimicrobial in either agar or broth media [20]. 

National Committee for Clinical Laboratory Standards, 1996, Method for Broth Dilution Antifungal Susceptibility Testing Yeast Approved Standard, M27-A, 15, 10, NCCLS, VA Medical Center, Tucson, AZ. 

National Committee for Clinical Laboratory Standards (1997) Reference method for broth dilution antifungal susceptibility testing of yeasts approved standard. NCCLS document M27-A, Wayne, PA. 

NCCLS (2002) Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts Approved Standard M-27-A2, 22 (15). National Committee on Clinical Laboratory Standards, Wayne, PA... 

TESTING FOR MICROBIAL SENSITIVITY TO ANTIMICROBIAL AGENTS Bacterial strains, even from the same species, may vary widely in sensitivity to antibiotics. Information about the antibiotic sensitivity of the infecting microorganism is important for appropriate drug selection. Various methods are used to assess susceptibility, including disk-diffusion, dilution test, and automated broth dilution. The results are either reported on a semi-quantitative scale i.e., resistant, intermediate, or susceptible) or in terms of the minimal inhibitory concentration (MIC). 

MICs) of a drug for comparison with anticipated blood or tissue levels. The two most common methods of susceptibility testing are disk dilfusion (Kirby-Bauer) and broth dilution. For some bacteria (eg, gonococci, enterococci, H influenzae), a direct test for beta-lac-tamase can be substituted, since susceptibility patterns are identical for all strains except for the production of beta-lactamase. 

Calgiani JN, Bartlett MS. EspineUlngrolf A, Ftomtling RA, Pfaller M, Rinaldi MG. Refer ence Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts Proposed Stan dard. NCCLS Document M27-T, Vol. 15, No. 10. Villanova, Pennsylvania NCCLS, 1995. 

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