Bronchoscopic instillation

The mean linear velocity of mucus, obtained from ten studies using instillation, was 14 5,5 mm/min, whereas that obtained from 13 studies using inhalation was 5.3 1.3 mm/min (2). The velocities measured after bronchoscopic instillation were higher and more variable than those measured after inhalation. This could be due to stimulation of the mucociliary transport system, either by... 

The routes of delivery of rAAV in animal models have been largely based upon the specific needs dictated by the disease process to be treated. For example, rAAV-cystic fibrosis transmembrane conductance regulator (CFTR) vectors that have been developed for treatment of cystic fibrosis (CF) were tested in New Zealand white rabbits and in rhesus macaques by the endobronchial route (Flotte et al., 1993 Afione et al., 1996 Conrad et al., 1996). In each instance aliquots of vector were instilled directly into the lumen of a bronchus through a fiberoptic bronchoscope. Vector DNA transfer and mRNA expression were detectable (albeit at low levels) for more than 6 months in each instance, without any indication of inflammation or any other toxicity. Studies in rhesus monkeys also indicated that the likelihood of rescue of rAAV by concomitant wild-type AAV and adenovirus infection was low. These studies... 

There are several possibihties for administration of drugs to the lung. One would be instillation of liquid direetly through a bronchoscope, or corresponding type instrument. Other ways that are commonly used in treatment are metered-dose... 

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