2-Bromo-2-methyl-propionic acid ethyl

Sodium 2-Bromo-2-methyl-propionic acid ethyl ester... 

The reaction of potassium 3-amino-4-oxo-3,4-dihydroquinazoline-2-thiolate 62 with a-bromophenylacetic acid 63 resulted in the formation of (3-amino-4-oxo-3,4-dihydroquinazolin-2-ylsulfanyl)-phenyl-acetic acid methyl ester 64 which on alkali treatment and subsequent acidification resulted in the synthesis of 2-phenyl- 1-thia-4,4a,9-triaza-anthracene-3,10-dione 65 <1999JCR(S)86>. Similarly, the reaction of potassium 3-amino-5,6-dimethyl-4-oxo-3,4,4a,7a-tetrahydrothieno[2,3- pyrimidine-2-thiolate 66 with a-bromo-ester 67 resulted in the formation of 2-(3-amino-5,6-dimethyl-4-oxo-3,4,4a,7a-tetrahydrothieno[2,3- / pyrimidin-2-ylsulfanyl)-propionic acid ethyl ester 68. Subsequent treatment with alkali followed by acidification resulted in the formation of 2,3,7-trimethyl-3a,9a-dihydro-l,8-dithia-4a,5,9-triazacyclopenta[ ]naphthalene-4,6-dione 69 <2000JHC1161>... 

In a more detailed study, the same esterase P. fluorescens) was again subjected to mutagenesis using the same mutator strain, but also by saturation mutagenesis at selected positions 133a). In addition to 3-phenylbutyric acid ethyl ester (27), 3-bromo-2-methyl-propionic acid methyl ester rac-31) was chosen for the hydrolytic kinetic resolution, with the WT PFE showing an E factor of 12 in favor of the (5)-32. 

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