Brine shrimp lethality assay

Hippocrateine I and II (52a,b) were first isolated from root and stem barks of Hippocratea excelsa[ 180], Structures of these compounds were established using FABMS, CIMS, and one-and two-dimensional 1H and NMR. The structure of 52a was confirmed by X-ray crystallography, and its absolute configuration determined by comparison with the known absolute stereochemistry of acanthothamine. Hippocrateine I (52a) was found to be slightly active in the brine shrimp lethality assay (LC50 = 212 pg/ml) and in the 9PS cytotoxicity assay (ED50 = 1.85 x 10"1 pg/ml), but it was inactive in the A-549, HT-29 and MCF-7 cell culture assays [180],... 

Two common in-house bioassays to screen for metabolites exhibiting general cytotoxicity, the fertilized sea urchin egg cytotoxicity assay and the brine shrimp lethality assay, were used to identify bioactive diterpene metabolites in two soft coral species collected from Sodwana Bay and Ponto do Ouro respectively. Sodwana Bay specimens of Sarcophyton... 

An ethanolic extract of the stem bark of Maytenus krukovii A. C. Smith demonstrated significant inhibitory activity in a protein kinase C (PKC) inhibition bioassay (IC50 10 ng/ml). The phytochemical study of this extract gave mayteine (61) and 6-benzoyl-6-deacetylmayteine (62), Fig. (20) both compounds showed toxicity in the brine shrimp lethality assay, with LC50 <10 lig/ml, but neither of them demonstrated significant PKC inhibitory activity, this was concentrated in non-alkaloidal fractions [167]. 

The LCgo of lemon balm essential oil in the brine shrimp lethality assay was 1279 pg/ml (Oliva et al. 2007). 

The LD50 of an ethanol extract of greater galangal in the brine shrimp lethality assay was 109 pg/ml (Khattak et al. 2005). 

In the brine shrimp lethality assay, the LC50 of various extracts of bacopa is 295 pg/ml for the ethanol extract, 5332 pg/ml for the water extract, 95 pg/ml for the saponin-rich... 

In the brine shrimp lethality assay, the LC50 of a methanol extract of cornflower is 38 pg/ml, while that of an ether extract of cornflower is 103 pg/ml (Janackovic et al. 2008). 

In the brine shrimp lethality assay, the LC50 values of compounds isolated from centaury are 8 ng/ml for swertiamarin, 34 pg/ml for sweroside, and 24 ng/ml for gentiopicroside (Kumarasamy et al. 2003a, 2003b). 

In the brine shrimp lethality assay, the LC50 of chelidonine was 319 ppm, and for prototropine was 49 ppm. LC50 values for ethanol and aqueous extracts of celandine could not be determined at concentrations up to 1000 ppm (Saglam and Arar 2003). 

In the brine shrimp lethality assay, the LD5d values of the major compounds from the methanol extract of wild carrot were 0.053mg/ml for luteolin, 1 mg/ml for luteolin 3-O-P-D-glucopyranoside, and 1 mg/ml for luteolin 4 -0-P-D-glucopyranoside (Kumarasamy et al. 2005). 

No toxic effects of flaxseed were observed in the brine shrimp lethality assay (Mahmoud et al. 1992). 

In the brine shrimp lethality assay, the LCjq of an alcohol extract of basil is 9.92 pg/ml (Lagarto Parra et al. 2001). 

Guided by the brine shrimp lethality assay, eight new glucocerebrosides (47-54) were obtained from the sponge Haliclona (Reniera) sp, (Fig, 17) [62], A novel feature of these cerebrosides was the presence of unprecedent... 

The antioxidant and anticancer evaluation of Scindapsus officinalis (Roxb.) Schott fruits has been attempted to investigate its antitumor activity [29]. The collection and authentication of the plant material, mainly fruits, and their various extractions were done. Identification of plant s active constituents by preliminary phytochemical screening was carried out. An in vitro cytotoxic assay using the brine shrimp lethality assay with brine shrimp eggs (Artemia salina) at a dose of 1-10 pg/ml with the fmit extract was performed. 

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